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Literature summary for 3.4.14.11 extracted from

  • De, A.; Lupidi, G.; Petrelli, D.; Vitali, L.
    Molecular cloning and biochemical characterization of Xaa-Pro dipeptidyl-peptidase from Streptococcus mutans and its inhibition by anti-human DPP IV drugs (2016), FEMS Microbiol. Lett., 363, fnw066 .
    View publication on PubMed
Search for more literature for 3.4.14.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Lactococcus lactis Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.1 mM, 83% residual activity Streptococcus mutans
Diprotin A
-
 Streptococcus mutans
o-phenanthroline 10 mM, 50% residual activity Streptococcus mutans
PMSF 1 mM, 37% residual activity Streptococcus mutans
saxagliptin inhibitor of human enzyme Streptococcus mutans
SDS 35 mM, no residual activity Streptococcus mutans
Zn2+ 0.1 mM, 57% residual activity Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.089
-
 Gly-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus mutans

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Streptococcus mutans Q8DVS2
-
-
Streptococcus mutans ATCC 700610 Q8DVS2
-
-

Purification (Commentary)

Purification (Comment) Organism
both native and recombinant protein Streptococcus mutans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1070
-
 pH 7.5, 37°C Streptococcus mutans

Storage Stability

Storage Stability Organism
-20°C, repeated freezing and thawing, 66% residaul activity Streptococcus mutans
-80°C, 3 months, 74% residual activity Streptococcus mutans
4°C, 10 times diluted enzyme, 24 h, loss of activity Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Pro-4-nitroanilide + H2O
-
 Streptococcus mutans Gly-Pro + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Streptococcus mutans ATCC 700610 Gly-Pro + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Streptococcus mutans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0167
-
 Diprotin A pH 7.5, 37°C Streptococcus mutans
0.129
-
 saxagliptin pH 7.5, 37°C Streptococcus mutans