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Literature summary for 3.4.14.11 extracted from

  • Perez-Guzman, A.E.; Cruz y Victoria, T.; Cruz-Camarillo, R.; Hernandez-Sanchez, H.
    Purification and characterization of X-prolyl-dipeptidyl aminopeptidase from Lactococcus lactis subsp. cremoris NRRL 634 (2006), World J. Microbiol. Biotechnol., 22, 953-958.
No PubMed abstract available
Search for more literature for 3.4.14.11

Inhibitors

Inhibitors Comment Organism Structure
Phenylmethanesulphonyl fluoride 0.1 mM, 20 min at 40°C, 40% loss of activity. 1 mM, complete inhibition after 20 min Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.92
-
 Arg-Pro-p-nitroanilide
-
 Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Lactococcus lactis 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 x * 82000, SDS-PAGE Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 subsp. cremoris NRRL 634
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Pro-p-nitroanilide + H2O
-
 Lactococcus lactis Arg-Pro + p-nitroaniline
-
 ?
Lys-Pro-p-nitroanilide + H2O 85% of the activity with Arg-Pro-p-nitroanilide Lactococcus lactis Lys-Pro + p-nitroaniline
-
 ?
additional information no activity with Pro-p-nitroanilide or azocasein Lactococcus lactis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Lactococcus lactis

Synonyms

Synonyms Comment Organism
Pep-XP
-
 Lactococcus lactis
X-prolyl-dipeptidyl aminopeptidase
-
 Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 substrate: Arg-Pro-p-nitroanilide Lactococcus lactis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 40°C: 95% of maximal activity, 50°C: 98% of maximal activity Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 substrate: Arg-Pro-p-nitroanilide Lactococcus lactis

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: 88% of maximal activity, pH 7.0: maximal activity Lactococcus lactis