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Literature summary for 3.4.14.11 extracted from

  • Sanz, Y.; Toldra, F.
    Purification and characterization of an X-prolyl-dipeptidyl peptidase from Lactobacillus sakei (2001), Appl. Environ. Microbiol., 67, 1815-1820.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.14.11

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin 1.0 mM, complete inhibition Latilactobacillus sakei
Cu2+ 1 mM CuCl2, 69% inhibition Latilactobacillus sakei
Hg2+ 1 mM HgCl2, 78% inhibition Latilactobacillus sakei
phenylmethylsulfonyl fluoride 1 mM, 43% inhibition Latilactobacillus sakei
Zn2+ 0.1 mM, 50% inhibition Latilactobacillus sakei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 Gly-Pro-7-amido-4-methylcoumarin pH 7.5, 37°C Latilactobacillus sakei
0.05
-
 Ala-Pro-4-nitroanilide pH 7.5, 37°C Latilactobacillus sakei
0.088
-
 Lys-Ala-7-amido-4-methylcoumarin pH 7.5, 37°C Latilactobacillus sakei
0.192
-
 Gly-Pro-4-nitroanilide pH 7.5, 37°C Latilactobacillus sakei

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not a metalloenzyme Latilactobacillus sakei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 2 * 88000, SDS-PAGE Latilactobacillus sakei
170000
-
 gel filtration Latilactobacillus sakei

Organism

Organism UniProt Comment Textmining
Latilactobacillus sakei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Latilactobacillus sakei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
236.5
-
-
 Latilactobacillus sakei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Pro 4-nitroanilide + H2O 60.5% of the activity with Gly-Pro-7-amido-4-methylcoumarin Latilactobacillus sakei Arg-Pro + 4-nitroaniline
-
 ?
Gly-Pro 4-nitroanilide + H2O 25.5% of the activity with Gly-Pro-7-amido-4-methylcoumarin Latilactobacillus sakei Gly-Pro + 4-nitroaniline
-
 ?
Gly-Pro-7-amido-4-methylcoumarin + H2O
-
 Latilactobacillus sakei Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
Gly-Pro-Ala + H2O
-
 Latilactobacillus sakei Gly-Pro + Ala
-
 ?
L-Ala-L-Pro-4-nitroanilide + H2O best substrate Latilactobacillus sakei L-Ala-L-Pro + 4-nitroaniline
-
 ?
Lys-Ala-7-amido-4-methylcoumarin + H2O 9.9% of the activity with Gly-Pro-7-amido-4-methylcoumarin Latilactobacillus sakei Lys-Ala + 7-amino-4-methylcoumarin
-
 ?
additional information maximal activity with Pro in the N-penultimate position Latilactobacillus sakei ?
-
 ?
Tyr-Pro-Phe + H2O
-
 Latilactobacillus sakei Tyr-Pro + Phe
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 88000, SDS-PAGE Latilactobacillus sakei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Latilactobacillus sakei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 65 about 30% of maximal activity at 20°C and at 65°C, hydrolysis of Gly-Pro-7-amido-4-methylcoumarin Latilactobacillus sakei
35 60 35°C: about 50% of maximal activity, 60°C: about 30% of maximal activity, hydrolysis of Gly-Pro-7-amido-4-methylcoumarin Latilactobacillus sakei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Latilactobacillus sakei