Literature summary for 3.4.14.11 extracted from

Vesanto, E.; Savijoki, K.; Rantanen, T.; Steele, J.L.; Palva, A.
An X-prolyl dipeptidyl aminopeptidase (pepX) gene from Lactobacillus helveticus (1995), Microbiology, 141, 3067-3075.

Search for more literature for 3.4.14.11

Cloned(Commentary)

Cloned (Comment)	Organism
-	Lactobacillus helveticus

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	not	Lactobacillus helveticus
Cu2+	-	Lactobacillus helveticus
EDTA	no inhibition	Lactobacillus helveticus
p-hydroxymercuribenzoate	reactivated by DTT	Lactobacillus helveticus
phenylmethylsulfonyl fluoride	-	Lactobacillus helveticus
Zn2+	-	Lactobacillus helveticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.28	-	Gly-Pro 4-nitroanilide	-	Lactobacillus helveticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90573	-	2 * 90573, calculation from nucleotide sequence	Lactobacillus helveticus
165000	-	gel filtration	Lactobacillus helveticus

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus helveticus	-	-	-
Lactobacillus helveticus 53/7	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lactobacillus helveticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
90	-	-	Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Arg-Pro 4-nitroanilide + H2O	-	Lactobacillus helveticus	Arg-Pro + 4-nitroaniline	-	?
Arg-Pro 4-nitroanilide + H2O	-	Lactobacillus helveticus 53/7	Arg-Pro + 4-nitroaniline	-	?
Gly-Pro 4-nitroanilide + H2O	-	Lactobacillus helveticus	Gly-Pro + 4-nitroaniline	-	?
Gly-Pro 4-nitroanilide + H2O	-	Lactobacillus helveticus 53/7	Gly-Pro + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 90573, calculation from nucleotide sequence	Lactobacillus helveticus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Lactobacillus helveticus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	50	about 55% of activity maximum at 10°C and 50°C	Lactobacillus helveticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Lactobacillus helveticus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	8	pH 5.5: about 50% of maximal activity, pH 8: about 65% of maximal activity	Lactobacillus helveticus

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Release 2023.1 (January 2023)