Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Lactobacillus helveticus | |
Cu2+ | - |
Lactobacillus helveticus | |
diisopropylfluorophosphate | - |
Lactobacillus helveticus | |
EDTA | - |
Lactobacillus helveticus | |
Hg2+ | - |
Lactobacillus helveticus | |
iodoacetate | - |
Lactobacillus helveticus | |
additional information | not: DTT, 1.0-19 mM | Lactobacillus helveticus | |
NEM | - |
Lactobacillus helveticus | |
PCMB | - |
Lactobacillus helveticus | |
phenylmethylsulfonyl fluoride | - |
Lactobacillus helveticus | |
Zn2+ | - |
Lactobacillus helveticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1-2.5 mM, 40°C, 15 min, activation | Lactobacillus helveticus | |
K+ | 10-50 mM, 40°C, 15 min, activation | Lactobacillus helveticus | |
Mg2+ | 2.5 mM, 40°C, 15 min, activation | Lactobacillus helveticus | |
Na+ | 10-50 mM, 40°C, 15 min, activation | Lactobacillus helveticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, native PAGE and SDS-PAGE | Lactobacillus helveticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus helveticus | - |
CNRZ 32 | - |
Lactobacillus helveticus CNRZ 32 | - |
CNRZ 32 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Lactobacillus helveticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala 4-nitroanilide + H2O | 2% of the activity with Gly-Pro 4-nitroanilide | Lactobacillus helveticus | Ala-Ala + 4-nitroaniline | - |
? | |
Ala-Pro-Gly + H2O | 11% of the activity with Gly-Pro 4-nitroanilide | Lactobacillus helveticus | Ala-Pro + Gly | - |
? | |
Arg-Pro 4-nitroanilide + H2O | 130% of the activity with Gly-Pro 4-nitroanilide | Lactobacillus helveticus | Arg-Pro + 4-nitroaniline | - |
? | |
Gly-Pro 4-nitroanilide + H2O | - |
Lactobacillus helveticus | Gly-Pro + 4-nitroaniline | - |
? | |
additional information | no activity with Ala-Leu-Ala | Lactobacillus helveticus | ? | - |
? | |
additional information | no activity with Ala-Leu-Ala | Lactobacillus helveticus CNRZ 32 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, native PAGE and SDS-PAGE | Lactobacillus helveticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lactobacillus helveticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 65 | 10°C: about 55% of activity maximum, 65°C: 34% of activity maximum | Lactobacillus helveticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Lactobacillus helveticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5: about 50% of maximal activity, pH 9: about 60% of maximal activity | Lactobacillus helveticus |