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Literature summary for 3.4.14.10 extracted from

  • Eklund, S.; Lindas, A.C.; Hamnevik, E.; Widersten, M.; Tomkinson, B.
    Exploring the active site of tripeptidyl-peptidase II through studies of pH dependence of reaction kinetics (2012), Biochim. Biophys. Acta, 1824, 561-570.
    View publication on PubMed
Search for more literature for 3.4.14.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Drosophila melanogaster
expressed in Escherichia coli Mus musculus
expressed in Escherichia coli Homo sapiens
expression in Escherichia coli Drosophila melanogaster
expression of murine TPP2 gene optimized for high-level expression in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
D387G mutant shows an increase in pKa for kcat Drosophila melanogaster
D387G mutant shows bell-shaped pH-dependence of kcat, possibly due to an impaired protonation of the leaving group Mus musculus
D387G the pKa values of mutant D387G for kcat (app) increase when Asp-387 is changed to glycine. Mutant shows a bell-shaped pH-dependence of kcat(app), possibly due to an impaired protonation of the leaving group. Mutant shows a decresed kcat for all substrates and a highly decreased kcat/Km compared to wild-type. In contrast to wild-type at higher pH the kcat (app) value decreases Drosophila melanogaster
E331Q mutant has a 2-3 orders of magnitude decreased kcat app/KM compared to wild-type, mostly dependent on an increase in KM Mus musculus
G375D less than 0.1% of wild-type activity Mus musculus
G375D mutant has only 0.1% activity of wild-type at pH 7.5. Mutant shows a marked decrease in pH optimum compared to wild-type Mus musculus
H267A mutant shows bell-shaped pH-dependence of kcat, possibly due to an impaired protonation of the leaving group Mus musculus
H267A mutant shows mostly a decresed kcat for all substrates and a highly decreased kcat/Km between 2 to fold orders of magnitude compared to wild-type. kcat shows a higher rise with pH in mutant H267A than in wild-type with substrate Ala-Ala-Phe-4-nitroanilide but at pH more than 7.6 kcat (app) decreases for substrate Ala-Ala-Phe-4-nitroanilide, resulting in a significantly better fit for a bell-shaped curve than for a sigmoidal Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
0.008
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Mus musculus
0.008
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
0.009
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Mus musculus
0.009
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
0.01
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
0.01
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Homo sapiens
0.01
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Mus musculus
0.01
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Mus musculus
0.01
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
0.01
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
0.01
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
0.0106
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.6, 37°C Homo sapiens
0.012
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
0.012
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Homo sapiens
0.012
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Homo sapiens
0.012
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.8, 37°C Homo sapiens
0.013
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Homo sapiens
0.013
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.4, 37°C Homo sapiens
0.013
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
0.013
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
0.014
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Mus musculus
0.014
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
0.015
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
0.015
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
0.017
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Mus musculus
0.017
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Homo sapiens
0.017
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.9, 37°C Homo sapiens
0.017
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
0.024
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Homo sapiens
0.024
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 8.3, 37°C Homo sapiens
0.024
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Mus musculus
0.024
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
0.028
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Mus musculus
0.028
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
0.03
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
0.03
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Homo sapiens
0.03
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.8, 37°C Homo sapiens
0.03
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
0.036
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
0.037
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Mus musculus
0.037
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
0.039
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
0.039
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
0.042
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Homo sapiens
0.042
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.4, 37°C Homo sapiens
0.05
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Homo sapiens
0.05
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.6, 37°C Homo sapiens
0.05
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
0.05
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
0.05
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
0.05
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
0.05
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
0.05
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
0.054
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Mus musculus
0.054
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
0.054
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
0.054
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
0.056
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
0.056
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
0.057
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
0.057
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
0.058
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
0.058
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
0.06
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Homo sapiens
0.06
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.9, 37°C Homo sapiens
0.06
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
0.06
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
0.06
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
0.06
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
0.062
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
0.062
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
0.062
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
0.062
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
0.07
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
0.07
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
0.074
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
0.074
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
0.09
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
0.09
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
0.09
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
0.09
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
0.1
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
0.1
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
0.11
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
0.11
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
0.12
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 8.3, 37°C Homo sapiens
0.12
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
0.13
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
0.13
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
0.2
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
0.2
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
0.21
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
0.21
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
0.25
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
0.25
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
0.27
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
0.27
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
0.3
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
0.33
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
0.5
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
0.6
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
0.6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
0.9
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
1.19
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
3.3
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus
5
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
20
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
30
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Drosophila melanogaster Q9V6K1
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Mus musculus Q64514
-
-

Purification (Commentary)

Purification (Comment) Organism
using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography Drosophila melanogaster
using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography Mus musculus
using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-
erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Ala-4-nitroanilide + H2O
-
 Drosophila melanogaster Ala-Ala-Ala + 4-nitroaniline
-
 ?
Ala-Ala-Ala-4-nitroanilide + H2O
-
 Mus musculus Ala-Ala-Ala + 4-nitroaniline
-
 ?
Ala-Ala-Ala-4-nitroanilide + H2O
-
 Homo sapiens Ala-Ala-Ala + 4-nitroaniline
-
 ?
Ala-Ala-Phe-4-nitroanilide + H2O
-
 Drosophila melanogaster Ala-Ala-Phe + 4-nitroaniline
-
 ?
Ala-Ala-Phe-4-nitroanilide + H2O
-
 Mus musculus Ala-Ala-Phe + 4-nitroaniline
-
 ?
Ala-Ala-Phe-4-nitroanilide + H2O
-
 Homo sapiens Ala-Ala-Phe + 4-nitroaniline
-
 ?
L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Homo sapiens L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?
L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Mus musculus L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?
L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Drosophila melanogaster L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?
L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Homo sapiens L-Ala-L-Ala-L-Phe + 4-nitroaniline
-
 ?
L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Mus musculus L-Ala-L-Ala-L-Phe + 4-nitroaniline
-
 ?
L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Drosophila melanogaster L-Ala-L-Ala-L-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 100000-140000, SDS-PAGE of recombinant protein, full-length TPP II and shortened versions thereof Mus musculus

Synonyms

Synonyms Comment Organism
dTPP II
-
 Drosophila melanogaster
hTPP II
-
 Homo sapiens
mTPP II
-
 Mus musculus
tripeptidyl aminopeptidase II
-
 Drosophila melanogaster
tripeptidyl aminopeptidase II
-
 Mus musculus
tripeptidyl aminopeptidase II
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Drosophila melanogaster
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
0.25
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
0.38
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
0.38
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
0.441
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
0.441
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
0.509
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
0.509
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
0.58
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
0.58
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
0.74
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
0.74
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
0.857
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
0.857
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
0.86
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
0.86
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
0.9
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
0.9
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
0.9
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
0.9
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
1
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
1
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
1.12
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
1.12
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
1.29
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
1.29
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
1.3
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
1.3
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
1.42
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
1.42
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
1.5
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
1.5
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
2
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Homo sapiens
2
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.4, 37°C Homo sapiens
3
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
3
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
3.1
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Homo sapiens
3.1
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.6, 37°C Homo sapiens
3.3
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Homo sapiens
3.3
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 8.3, 37°C Homo sapiens
4
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Homo sapiens
4
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.8, 37°C Homo sapiens
4
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
4
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
4.2
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
5
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Homo sapiens
5
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.9, 37°C Homo sapiens
5
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
5
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
6
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
7.2
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
7.2
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
9.6
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Homo sapiens
9.6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.4, 37°C Homo sapiens
10
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Homo sapiens
10
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.8, 37°C Homo sapiens
10
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus
10
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
11
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
11
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
11
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
12
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
12
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
12
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
12
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
13
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
13
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
13
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
13
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
18
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
19
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Homo sapiens
19
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.9, 37°C Homo sapiens
21
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Mus musculus
21
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
21
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Mus musculus
22
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Homo sapiens
22
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.6, 37°C Homo sapiens
27
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Homo sapiens
27
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 8.3, 37°C Homo sapiens
27
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Mus musculus
27
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
30
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Mus musculus
30
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
33
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Mus musculus
33
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Mus musculus
33
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
33
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
38
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
38
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
42
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
42
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Mus musculus
50
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
50
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
56.8
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Mus musculus
57
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
57
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
58.8
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
60
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
60
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
65
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
65
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
80
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
96
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Mus musculus
96
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
100
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
114
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Mus musculus
114
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
125
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
500
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Drosophila melanogaster
7.5
-
 assay at Mus musculus
7.5
-
 assay at Homo sapiens
7.6
-
 optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide Drosophila melanogaster
7.9
-
 optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide Mus musculus
7.9
-
 optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH Homo sapiens
additional information
-
 isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH Mus musculus
additional information
-
 isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH Drosophila melanogaster
additional information
-
 kcat(app) increases with pH Drosophila melanogaster
additional information
-
 kcat(app) increases with pH Mus musculus
additional information
-
 kcat(app) increases with pH Homo sapiens
7 8 optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide Drosophila melanogaster
7 8 optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide Mus musculus
7 8 optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0000038
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
0.000005
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
0.000006
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
0.000006
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
0.000007
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
0.00001
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
0.00001
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
0.000011
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
0.000012
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
0.000013
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
0.000014
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
0.0000165
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
0.00002
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
0.000021
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
0.000021
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
0.000022
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
0.000023
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
0.00003
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
0.00003
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
0.000036
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
0.000138
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Homo sapiens
0.00016
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Homo sapiens
0.00019
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
0.00023
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Mus musculus
0.00023
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Homo sapiens
0.00025
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Homo sapiens
0.00028
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Homo sapiens
0.0003
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Homo sapiens
0.0003
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Homo sapiens
0.00031
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Homo sapiens
0.00033
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Homo sapiens
0.00033
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
0.00037
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Homo sapiens
0.0005
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
0.00056
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
0.00056
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
0.00082
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
0.0009
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
0.00098
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
0.001
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
0.0015
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
0.0018
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Mus musculus
0.0025
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Mus musculus
0.0026
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Mus musculus
0.0028
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Mus musculus
0.0029
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Mus musculus
0.0031
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Mus musculus
0.0035
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Mus musculus
0.0038
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Mus musculus
0.0039
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Mus musculus
3.4
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
3.82
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
5
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
6
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
6.7
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
7.1
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
8
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
9
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus
9.6
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
10
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
10
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
11
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
12
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
13
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
14
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
15
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus
15
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
16.5
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
17
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
20
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
20
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
21
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
21
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
22
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
23
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
30
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
30
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
36
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
138
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.4, 37°C Homo sapiens
160
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 8.3, 37°C Homo sapiens
190
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
230
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.4, 37°C Homo sapiens
250
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 8.3, 37°C Homo sapiens
280
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.6, 37°C Homo sapiens
300
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.9, 37°C Homo sapiens
300
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.8, 37°C Homo sapiens
310
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.8, 37°C Homo sapiens
330
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.9, 37°C Homo sapiens
330
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
370
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.6, 37°C Homo sapiens
500
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
560
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
560
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
820
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
900
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
980
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
1000
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
1500
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
1800
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
2100
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
2500
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
2600
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
2800
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
2900
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
3100
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
3500
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
3800
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
3900
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus