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Literature summary for 3.4.14.10 extracted from

  • Balöw, R.M.; Ragnarsson, U.; Zetterquist, Ö.
    Tripeptidyl aminopeptidase in the extralysosomal fraction of rat liver (1983), J. Biol. Chem., 258, 11622-11628.
    View publication on PubMed
Search for more literature for 3.4.14.10

General Stability

General Stability Organism
dithiothreitol and glycerol stabilize Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Arg-Arg-Ala-(phospho)Ser-Val-Ala inhibits cleavage of Leu-Arg bond, Gly-Val-Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala as substrate Rattus norvegicus
Guanidinovaleric acid-Arg-Ala-(phospho)Ser-Val
-
 Rattus norvegicus
Leu-Arg-Arg-Ala-(phospho)Ser-Val weak, Gly-Val-Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala as substrate Rattus norvegicus
Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala weak, Gly-Val-Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala as substrate Rattus norvegicus
Val-Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala hydrolysis of Gly-Val-Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 Arg-Arg-Ala-(phospho)Ser-Val-Ala pH 6.5 Rattus norvegicus
0.015
-
 Arg-Arg-Ala-(phospho)Ser-Val-Ala pH 7.5 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1000000
-
 value above, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, in microsomal extract, 3 months Rattus norvegicus
4°C, in microsomal extract, at least 3 days, less stable in phosphate buffer diluted extracts Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Arg-Ala-(phospho)Ser-Val + H2O the Ala-(phospho)Ser bond is cleaved at the same rate as that of Arg-Arg-Ala-(phospho)Ser-Val-Ala Rattus norvegicus ?
-
 ?
Arg-Arg-Ala-(phospho)Ser-Val-Ala + H2O
-
 Rattus norvegicus Arg-Arg-Ala + (phospho)Ser-Val-Ala
-
 ?
Arg-Arg-Ala-Ser-Val + H2O
-
 Rattus norvegicus Arg-Arg-Ala + Ser-Val
-
 ?
Arg-Arg-Ala-Ser-Val-Ala + H2O better substrate than the phosphorylated peptide Rattus norvegicus Arg-Arg-Ala + Ser-Val-Ala
-
 ?
Gly-Val-Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala + H2O major cleavage site: L-Arg, followed by cleavage at Ala-(phospho)Ser Rattus norvegicus Gly-Val-Leu + Arg-Arg-Ala-(phospho)Ser-Val-Ala
-
 ?
Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala + H2O poor substrate Rattus norvegicus Leu-Arg-Arg + Ala-(phospho)Ser-Val-Ala
-
 ?
Lys-Arg-Ala-Ser-Val + H2O
-
 Rattus norvegicus Lys-Arg-Ala + Ser-Val
-
 ?
additional information requires free amino terminus Rattus norvegicus ?
-
 ?
additional information no substrates are Val-Leu-Arg-Arg-Ala-(phospho)Ser-Val-Ala, guanidovaleric acid-Arg-Arg-Ala-(phospho)Ser-Val, guanidovaleric acid-Arg-Arg-Ala-Ser-Val, epsilon-aminohexanoic acid-Arg-Arg-Ala-Ser-Val Rattus norvegicus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 hydrolysis of Arg-Arg-Ala-(phospho)Ser-Val-Ala Rattus norvegicus