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Literature summary for 3.4.14.1 extracted from

  • Legowska, M.; Hamon, Y.; Wojtysiak, A.; Grzywa, R.; Sie?czyk, M.; Burster, T.; Korkmaz, B.; Lesner, A.
    Development of the first internally-quenched fluorescent substrates of human cathepsin C The application in the enzyme detection in biological samples (2016), Arch. Biochem. Biophys., 612, 91-102 .
    View publication on PubMed

Application

Application Comment Organism
analysis application of substrate beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2) for detection of cathepsin C activity in various cell lysates, urine and bronchoalveolar lavage fluid samples Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular docking of substrate beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2). The interactions between the amino-terminal group of the substrate and the Asp1 and Gly277 of Cat C are responsible for the substrate N-terminus docking and are crucial for proteolytic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens
0.003
-
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C Homo sapiens
0.0032
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens
0.0032
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens
0.0034
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens
0.0036
-
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin pH 6.0, 37°C Homo sapiens
0.0052
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens
0.0052
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P53634
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 + H2O specific and selective substrate Homo sapiens beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala + Ser-Gln-Tyr(3-NO2)-NH2
-
?
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 + H2O specific and selective substrate Homo sapiens beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala + Ser-Gly-Tyr(3-NO2)-NH2
-
?
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 + H2O
-
Homo sapiens beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala + Ser-Tyr(3-NO2)-NH2
-
?
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin + H2O
-
Homo sapiens beta-(2-thienyl)Ala-Phe + 7-amino-4-methylcoumarin
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.83
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens
2.07
-
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C Homo sapiens
2.51
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens
3.45
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens
3.5
-
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin pH 6.0, 37°C Homo sapiens
4.29
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens
5.45
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens
6.19
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
491
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens
560
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens
684
-
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C Homo sapiens
964
-
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin pH 6.0, 37°C Homo sapiens
1180
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens
1340
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 pH 5.0, 37°C Homo sapiens
1560
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens
1660
-
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 pH 6.0, 37°C Homo sapiens