Application | Comment | Organism |
---|---|---|
analysis | application of substrate beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2) for detection of cathepsin C activity in various cell lysates, urine and bronchoalveolar lavage fluid samples | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
molecular docking of substrate beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2). The interactions between the amino-terminal group of the substrate and the Asp1 and Gly277 of Cat C are responsible for the substrate N-terminus docking and are crucial for proteolytic activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens | |
0.003 | - |
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C | Homo sapiens | |
0.0032 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens | |
0.0032 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens | |
0.0034 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens | |
0.0036 | - |
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin | pH 6.0, 37°C | Homo sapiens | |
0.0052 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens | |
0.0052 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53634 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 + H2O | specific and selective substrate | Homo sapiens | beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala + Ser-Gln-Tyr(3-NO2)-NH2 | - |
? | |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 + H2O | specific and selective substrate | Homo sapiens | beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala + Ser-Gly-Tyr(3-NO2)-NH2 | - |
? | |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 + H2O | - |
Homo sapiens | beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala + Ser-Tyr(3-NO2)-NH2 | - |
? | |
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | beta-(2-thienyl)Ala-Phe + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.83 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens | |
2.07 | - |
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C | Homo sapiens | |
2.51 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens | |
3.45 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens | |
3.5 | - |
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin | pH 6.0, 37°C | Homo sapiens | |
4.29 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens | |
5.45 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens | |
6.19 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
491 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens | |
560 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens | |
684 | - |
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C | Homo sapiens | |
964 | - |
beta-(2-thienyl)Ala-Phe-7-amido-4-methylcoumarin | pH 6.0, 37°C | Homo sapiens | |
1180 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens | |
1340 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 | pH 5.0, 37°C | Homo sapiens | |
1560 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gly-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens | |
1660 | - |
beta-(2-thienyl)Ala-beta-(7-methoxycoumarin-4-yl)Ala-Ser-Gln-Tyr(3-NO2)-NH2 | pH 6.0, 37°C | Homo sapiens |