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Literature summary for 3.4.14.1 extracted from
- Dipeptidyl peptidase I from goat brain: purification, characterization and its action on Leu-enkephalin (1993), Neurochem. Int., 22, 59-68.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithioerythritol | 5 mM, activates | Capra hircus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Capra hircus | |
| iodoacetamide | - |
Capra hircus | |
| iodoacetate | - |
Capra hircus | |
| L-trans-epoxy-succinyl-leucylamido(4-guanidino)-butane | - |
Capra hircus | |
| leupeptin | - |
Capra hircus | |
| NEM | - |
Capra hircus | |
| PCMB | - |
Capra hircus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
Gly-Arg-2-naphthylamide | - |
Capra hircus | |
| 0.14 | - |
Gly-Arg-4-methoxy-beta-naphthylamide | - |
Capra hircus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | 20 mM, required for maximal activity | Capra hircus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
x * 22000, SDS-PAGE in presence of 2-mercaptoethanol | Capra hircus |
| 180000 | - |
gel filtration | Capra hircus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Capra hircus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Capra hircus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Capra hircus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.019 | - |
- |
Capra hircus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gly-Arg-2-naphthylamide + H2O | - |
Capra hircus | Gly-Arg + 2-naphthylamine | - |
? | |
| Gly-Arg-4-methoxy-2-naphthylamide + H2O | - |
Capra hircus | Gly-Arg + 4-methoxy-2-naphthylamine | - |
? | |
| Leu-enkephalin + H2O | i.e. Tyr-Gly-Gly-Phe-Leu | Capra hircus | Tyr-Gly + Gly-Phe-Leu | no further degradation of the tripeptide | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22000, SDS-PAGE in presence of 2-mercaptoethanol | Capra hircus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Capra hircus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
at pH 4.6, stable up to | Capra hircus |
| 60 | - |
at pH 5.5, stable up to | Capra hircus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
Gly-Arg-4-methoxy-beta-naphthylamide as substrate | Capra hircus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | stable | Capra hircus |
| 4.6 | - |
stable up to 50°C | Capra hircus |
| 5.5 | - |
stable up to 60°C | Capra hircus |
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