Cloned (Comment) | Organism |
---|---|
gene PH0974, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 (lambdaDE3) | Pyrococcus horikoshii |
gene PH1149, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 (lambdaDE3) | Pyrococcus horikoshii |
gene PH1902, DNA and amino acid sequence determination and analysis | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii | |
3.4 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus horikoshii | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | dependent on | Pyrococcus horikoshii | |
Cu2+ | activates | Pyrococcus horikoshii | |
Fe2+ | activates | Pyrococcus horikoshii | |
Mn2+ | activates | Pyrococcus horikoshii | |
Ni2+ | activates | Pyrococcus horikoshii | |
Zn2+ | activates | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58885 | PH1149 or PepQ; isozyme PH1149 | - |
Pyrococcus horikoshii | O59565 | PH1902; isozyme PH1902 | - |
Pyrococcus horikoshii | P81535 | PH0974 or PepQ; gene pepQ, isozyme PH0974 or PepQ | - |
Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | O58885 | PH1149 or PepQ; isozyme PH1149 | - |
Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | P81535 | PH0974 or PepQ; gene pepQ, isozyme PH0974 or PepQ | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate and metal specificities, recombinant enzyme, overview | Pyrococcus horikoshii |
1938 | - |
purified recombinant enzyme, 100°C, substrate Met-Pro | Pyrococcus horikoshii |
2355 | - |
purified recombinant enzyme, 100°C, substrate Met-Pro | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro + H2O | - |
Pyrococcus horikoshii | Ala + Pro | - |
? | |
Arg-Pro + H2O | - |
Pyrococcus horikoshii | Arg + Pro | - |
? | |
Arg-Pro + H2O | - |
Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | Arg + Pro | - |
? | |
Gly-Pro + H2O | - |
Pyrococcus horikoshii | Gly + Pro | - |
? | |
Leu-Pro + H2O | high activity | Pyrococcus horikoshii | Leu + Pro | - |
? | |
Leu-Pro + H2O | high activity | Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | Leu + Pro | - |
? | |
Met-Pro + H2O | best substrate | Pyrococcus horikoshii | Met + Pro | - |
? | |
Met-Pro + H2O | best substrate | Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | Met + Pro | - |
? | |
additional information | substrate specificity, overview | Pyrococcus horikoshii | ? | - |
? | |
additional information | substrate specificity, overview | Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | ? | - |
? | |
Phe-Pro + H2O | - |
Pyrococcus horikoshii | Phe + Pro | - |
? | |
Pro-Ala + H2O | very low activity | Pyrococcus horikoshii | Pro + Ala | - |
? | |
Pro-Ala + H2O | very low activity | Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 / 700860D-5 | Pro + Ala | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant enzyme, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
PepQ | - |
Pyrococcus horikoshii |
PH0974 | - |
Pyrococcus horikoshii |
PH1149 | - |
Pyrococcus horikoshii |
Ph1prol | - |
Pyrococcus horikoshii |
Phprol | - |
Pyrococcus horikoshii |
prolidase | - |
Pyrococcus horikoshii |
prolidase homolog 1 | - |
Pyrococcus horikoshii |
prolidase homolog 2 | - |
Pyrococcus horikoshii |
Proline dipeptidase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 100 | assay at | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
purified recombinant enzyme, t1/2 is 21 h | Pyrococcus horikoshii |
90 | - |
purified recombinant enzyme, t1/2 is 21.5 h | Pyrococcus horikoshii |
100 | - |
purified recombinant enzyme, 8 h, 100% stable | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2617 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii | |
3812 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
two peaks at pH 5.0 and pH 7.0 | Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity profile, overview | Pyrococcus horikoshii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
768 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii | |
2006 | - |
Met-Pro | pH 7.0, 100°C | Pyrococcus horikoshii |