Literature summary for 3.4.13.9 extracted from

Lupi, A.; Della Torre, S.; Campari, E.; Tenni, R.; Cetta, G.; Rossi, A.; Forlino, A.
Human recombinant prolidase from eukaryotic and prokaryotic sources. Expression, purification, characterization and long-term stability studies (2006), FEBS J., 273, 5466-5478.

Search for more literature for 3.4.13.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21 (DE3) under the control of the cytomegalovirus promoter and the SP163 enhancer	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme decficiency leads to a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract, genotype/phenotype relationship, overview	Homo sapiens

General Stability

General Stability	Organism
Mn2+ and GSH stabilize the untagged recombinant enzyme	Homo sapiens
PEG and glycerol stabilize the enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
N-Benzyloxycarbonyl-L-proline	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	can substitute for Mn2+ by less than 30%	Homo sapiens
Co2+	can substitute for Mn2+ by less than 30%	Homo sapiens
Mg2+	can substitute for Mn2+ by less than 30%	Homo sapiens
Mn2+	dependent on, best divalent cation	Homo sapiens
Zn2+	can substitute for Mn2+ by less than 30%	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	x * 58000, recombinant enzyme, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	prolidase deficiency causes a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity, on-column digestion of the N-terminal His-tag by factor Xa	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
hydrolysis of Xaa-/-Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro-Pro	catalytic mechanism	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
fibroblast	-	Homo sapiens	-

Storage Stability

Storage Stability	Organism
37°C, purified recombinant enzyme, loss of almost all activity after 2 days, 70% remaining activity after one day for the detagged enzyme, 47% remaining activity for the tagged enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ala-Pro + H2O	-	Homo sapiens	Ala + Pro	-	?
Gly-Pro + H2O	-	Homo sapiens	Gly + Pro	-	?
Leu-Pro + H2O	-	Homo sapiens	Leu + Pro	-	?
additional information	prolidase deficiency causes a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract	Homo sapiens	?	-	?
additional information	prolidase is a Mn2+-dependent exo- or dipeptidase that cleaves imidodipeptides containing C-terminal proline or hydroxyproline, substrate specificity of native and recombinant enzymes, molecular modeling, overview	Homo sapiens	?	-	?
Phe-Pro + H2O	-	Homo sapiens	Phe + Pro	-	?

Subunits

Subunits	Comment	Organism
?	x * 58000, recombinant enzyme, SDS-PAGE	Homo sapiens
More	molecular modeling	Homo sapiens

Synonyms

Synonyms	Comment	Organism
More	the enzyme is a member of the metallopeptidase family	Homo sapiens
prolidase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
37	50	50% of maximal activity at 37°C	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	-	Homo sapiens

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Release 2023.1 (January 2023)