Literature summary for 3.4.13.9 extracted from

  • Lee, H.J.; Chen, Y.S.; Chou, C.Y.; Chien, C.H.; Lin, C.H.; Chang, G.G.; Chen, X.
    Investigation of the dimer interface and substrate specificity of prolyl dipeptidase DPP8 (2006), J. Biol. Chem., 281, 38653-38662.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in HEK-293T cells, expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system Homo sapiens

Engineering

Protein Variants Comment Organism
H859A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
V833A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
F822A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
Y844A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and substrate specificity of wild-type and mutant enzymes, overview Homo sapiens
2.72
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant H859A Homo sapiens
0.127
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged wild-type enzyme Homo sapiens
1.495
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant F822A Homo sapiens
2.219
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant V833A Homo sapiens
2.397
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant Y844A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
hydrodynamic properties of wild-type and mutant DPP8s Homo sapiens
210000
-
analytical ultracentrifugation Homo sapiens
105000
-
2 * 105000, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from HEK-293T cells by nickel affinity and anion exchange chromatography, followed by gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of wild-type and mutant enzymes, overview Homo sapiens ?
-
?
Arg-Pro + H2O preferred substrate Homo sapiens Arg + Pro
-
?
Leu-Pro + H2O
-
Homo sapiens Leu + Pro
-
?
Met-Pro + H2O high activity Homo sapiens Met + Pro
-
?
Ser-Pro + H2O
-
Homo sapiens Ser + Pro
-
?
Gly-Pro + H2O
-
Homo sapiens Gly + Pro
-
?
Ala-Pro + H2O
-
Homo sapiens Ala + Pro
-
?
Asp-Pro + H2O
-
Homo sapiens Asp + Pro
-
?
Lys-Pro + H2O
-
Homo sapiens Lys + Pro
-
?

Subunits

Subunits Comment Organism
dimer 2 * 105000, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens
More hydrodynamic properties of wild-type and mutant DPP8s, overview Homo sapiens

Synonyms

Synonyms Comment Organism
More DPP8 belongs to the family of prolyl dipeptidases Homo sapiens
prolyl dipeptidase
-
Homo sapiens
DPP8
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutants F822A and Y844A Homo sapiens
1
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant V833A Homo sapiens
8
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant H859A Homo sapiens
22
-
Ala-Pro pH 7.8, 37°C, recombinant His-tagged wild-type enzyme Homo sapiens