Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in HEK-293T cells, expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F822A | site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme | Homo sapiens |
H859A | site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme | Homo sapiens |
V833A | site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme | Homo sapiens |
Y844A | site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and substrate specificity of wild-type and mutant enzymes, overview | Homo sapiens | |
0.127 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged wild-type enzyme | Homo sapiens | |
1.495 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged mutant F822A | Homo sapiens | |
2.219 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged mutant V833A | Homo sapiens | |
2.397 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged mutant Y844A | Homo sapiens | |
2.72 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged mutant H859A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
hydrodynamic properties of wild-type and mutant DPP8s | Homo sapiens |
105000 | - |
2 * 105000, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
210000 | - |
analytical ultracentrifugation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from HEK-293T cells by nickel affinity and anion exchange chromatography, followed by gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro + H2O | - |
Homo sapiens | Ala + Pro | - |
? | |
Arg-Pro + H2O | preferred substrate | Homo sapiens | Arg + Pro | - |
? | |
Asp-Pro + H2O | - |
Homo sapiens | Asp + Pro | - |
? | |
Gly-Pro + H2O | - |
Homo sapiens | Gly + Pro | - |
? | |
Leu-Pro + H2O | - |
Homo sapiens | Leu + Pro | - |
? | |
Lys-Pro + H2O | - |
Homo sapiens | Lys + Pro | - |
? | |
Met-Pro + H2O | high activity | Homo sapiens | Met + Pro | - |
? | |
additional information | substrate specificity of wild-type and mutant enzymes, overview | Homo sapiens | ? | - |
? | |
Ser-Pro + H2O | - |
Homo sapiens | Ser + Pro | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 105000, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
More | hydrodynamic properties of wild-type and mutant DPP8s, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DPP8 | - |
Homo sapiens |
More | DPP8 belongs to the family of prolyl dipeptidases | Homo sapiens |
prolyl dipeptidase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged mutants F822A and Y844A | Homo sapiens | |
1 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged mutant V833A | Homo sapiens | |
8 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged mutant H859A | Homo sapiens | |
22 | - |
Ala-Pro | pH 7.8, 37°C, recombinant His-tagged wild-type enzyme | Homo sapiens |