Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.13.9 extracted from

  • Ghosh, M.; Grunden, A.M.; Dunn, D.M.; Weiss, R.; Adams, M.W.W.
    Characterization of native and recombinant forms of an unusual cobalt-dependent proline dipeptidase (prolidase) from the hyperthermophilic archaeon Pyrococcus furiosus (1998), J. Bacteriol., 180, 4781-4789.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.13.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 Met-Pro native prolidase Pyrococcus furiosus
3
-
 Leu-Pro native prolidase Pyrococcus furiosus
3.3
-
 Met-Pro recombinant prolidase Pyrococcus furiosus
4.2
-
 Val-Pro native prolidase Pyrococcus furiosus
8.3
-
 Ala-Pro native prolidase Pyrococcus furiosus
20
-
 Phe-Pro native prolidase Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requires Co2+ in addition to one cobalt atom bound per subunit Pyrococcus furiosus
Mn2+ Co2+ can be replaced by Mn2+ Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Pyrococcus furiosus
100000
-
 gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant form Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
630
-
 native enzyme Pyrococcus furiosus
1360
-
 recombinant enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-Pro + H2O
-
 Pyrococcus furiosus L-Ala + Pro
-
 ?
L-Phe-Pro + H2O
-
 Pyrococcus furiosus L-Phe + Pro
-
 ?
L-Val-Pro + H2O
-
 Pyrococcus furiosus L-Val + Pro
-
 ?
Leu-Pro + H2O
-
 Pyrococcus furiosus Leu + Pro
-
 ?
Met-Pro + H2O
-
 Pyrococcus furiosus Met + Pro
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
 substrate Met-Pro Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 substrate Met-Pro Pyrococcus furiosus