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Literature summary for 3.4.13.22 extracted from

  • Deghorain, M.; Goffin, P.; Fontaine, L.; Mainardi, J.L.; Daniel, R.; Errington, J.; Hallet, B.; Hols, P.
    Selectivity for D-lactate incorporation into the peptidoglycan precursors of Lactobacillus plantarum: role of Aad, a VanX-like D-alanyl-D-alanine dipeptidase (2007), J. Bacteriol., 189, 4332-4337.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.13.22

Cloned(Commentary)

Cloned (Comment) Organism
overexpression is achieved by cloning aad under the control of the nisin-inducible promoter PnisA from Lactococcus lactis. The resulting plasmid (pGIM023) is introduced into Lactobacillus plantarum strain NZ7100, a WCFS1 derivative carrying the nisR and nisK regulatory genes required for nisin induction Lactiplantibacillus plantarum

Protein Variants

Protein Variants Comment Organism
additional information an aad knockout mutant (MD119) is constructed in NZ7100 using a two-step homologous recombination procedure. Aad inactivation results in the stable replacement of the aad open reading frame (encoding 180 amino acid residues out of 185) by an erythromycin resistance cassette Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no detectable activity in aad knockout mutant (MD119) Lactiplantibacillus plantarum
0.0015
-
 in strain overexpressing aad (NZ7100(pGIM023)), in the absence of 15 mM ZnCl2 Lactiplantibacillus plantarum
0.0019
-
 wild type strain NZ7100 , in the presence of 15 mM ZnCl2 Lactiplantibacillus plantarum
0.3872
-
 in strain overexpressing aad (NZ7100(pGIM023)), in the presence of 15 mM ZnCl2 Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-D-Ala + H2O
-
 Lactiplantibacillus plantarum D-Ala + D-Ala
-
 ?

Synonyms

Synonyms Comment Organism
AAD
-
 Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Lactiplantibacillus plantarum