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Literature summary for 3.4.13.20 extracted from

  • Heck, T.; Limbach, M.; Geueke, B.; Zacharias, M.; Gardiner, J.; Kohler, H.P.; Seebach, D.
    Enzymatic degradation of beta- and mixed alpha,beta-oligopeptides (2006), Chem. Biodivers., 3, 1325-1348.
    View publication on PubMed
Search for more literature for 3.4.13.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brucella anthropi

Crystallization (Commentary)

Crystallization (Comment) Organism
active-site modeling of DmpA. Based on the crystal structure and the catalytic mechanism proposed for DmpA, carnosine is manually introduced into the active site of the enzyme. The model shows that carnosine fits excellently, in line with the high specific activity for this substrate Brucella anthropi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
 beta-Ala-4-nitroanilide pH 8.0, 25°C Brucella anthropi
0.019
-
 beta-homoAla-4-nitroanilide pH 8.0, 25°C Brucella anthropi
1.1
-
 beta-homoPhe-4-nitroanilide pH 8.0, 25°C Brucella anthropi

Organism

Organism UniProt Comment Textmining
Brucella anthropi Q59632
-
-
Brucella anthropi LMG7991 Q59632
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brucella anthropi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O DmpA cleaves the N-terminal beta-homoAla, no further hydrolysis is observed within 13 days Brucella anthropi beta-homoAla + beta-homoLys-beta-homoPhe-beta-homoAla-beta-homoLys-beta-homoPhe
-
 ?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O DmpA cleaves the N-terminal beta-homoAla, no further hydrolysis is observed within 13 days Brucella anthropi LMG7991 beta-homoAla + beta-homoLys-beta-homoPhe-beta-homoAla-beta-homoLys-beta-homoPhe
-
 ?
beta-Ala-4-nitroanilide + H2O
-
 Brucella anthropi beta-Ala + 4-nitroaniline
-
 ?
beta-Ala-4-nitroanilide + H2O
-
 Brucella anthropi LMG7991 beta-Ala + 4-nitroaniline
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O hydrolysis at 1.3% compared to hydrolysis of carnosine Brucella anthropi beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O hydrolysis at 1.3% compared to hydrolysis of carnosine Brucella anthropi LMG7991 beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-L-His + H2O i.e. carnosine, preferred substrate Brucella anthropi beta-Ala + L-His
-
 ?
beta-Ala-L-His + H2O i.e. carnosine, preferred substrate Brucella anthropi LMG7991 beta-Ala + L-His
-
 ?
beta-homoAla-4-nitroanilide + H2O
-
 Brucella anthropi beta-homoAla + 4-nitroaniline
-
 ?
beta-homoAla-beta-homoLeu + H2O hydrolysis at 55% compared to hydrolysis of carnosine Brucella anthropi beta-homoAla + beta-homoLeu
-
 ?
beta-homoLeu-Ile-beta-homoTyr + H2O hydrolysis at 0.01% compared to hydrolysis of carnosine Brucella anthropi beta-homoLeu + Ile-beta-homoTyr
-
 ?
beta-homoPhe-4-nitroanilide + H2O
-
 Brucella anthropi beta-homoPhe + 4-nitroaniline
-
 ?
beta-homoSer-Ile-beta-homoTyr + H2O hydrolysis at 0.06% compared to hydrolysis of carnosine Brucella anthropi beta-homoSer + Ile-beta-homoTyr
-
 ?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O hydrolysis at 0.19% compared to hydrolysis of carnosine Brucella anthropi beta-homoVal + beta-homoAla-beta-homoLeu
-
 ?
beta-homoVal-Ile-beta-homoTyr + H2O hydrolysis at 0.01% compared to hydrolysis of carnosine Brucella anthropi beta-homoVal + Ile-beta-homoTyr
-
 ?
beta-homoVal-Ile-Tyr + H2O hydrolysis at 0.09% compared to hydrolysis of carnosine Brucella anthropi beta-homoVal + Ile-Tyr
-
 ?
additional information cleaves beta- and mixed alpha,beta-peptides and amides, but a short side chain of the N-terminal beta-amino acid residue seems to be a prerequisite, since only peptides carrying N-terminal betahGly and betahAla are hydrolyzed with good efficiencies. Tripeptides carrying two consecutive N-terminal beta-homoamino acids as well as peptides with alpha-amino acids in the N-terminal position do not serve as substrates for DmpA Brucella anthropi ?
-
 ?
additional information cleaves beta- and mixed alpha,beta-peptides and amides, but a short side chain of the N-terminal beta-amino acid residue seems to be a prerequisite, since only peptides carrying N-terminal betahGly and betahAla are hydrolyzed with good efficiencies. Tripeptides carrying two consecutive N-terminal beta-homoamino acids as well as peptides with alpha-amino acids in the N-terminal position do not serve as substrates for DmpA Brucella anthropi LMG7991 ?
-
 ?

Synonyms

Synonyms Comment Organism
DmpA
-
 Brucella anthropi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Brucella anthropi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 beta-homoPhe-4-nitroanilide pH 8.0, 25°C Brucella anthropi
30.5
-
 beta-Ala-4-nitroanilide pH 8.0, 25°C Brucella anthropi
51.4
-
 beta-homoAla-4-nitroanilide pH 8.0, 25°C Brucella anthropi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Brucella anthropi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.027
-
 beta-homoPhe-4-nitroanilide pH 8.0, 25°C Brucella anthropi
2600
-
 beta-homoAla-4-nitroanilide pH 8.0, 25°C Brucella anthropi
7800
-
 beta-Ala-4-nitroanilide pH 8.0, 25°C Brucella anthropi