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Literature summary for 3.4.13.19 extracted from
- Purification and characterization of dipeptidase hydrolyzing L-cysteinylglycine from radish cotyledon (2007), Biosci. Biotechnol. Biochem., 71, 3102-3104.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 87% inhibition at 1 mM | Raphanus sativus |  |
| N-ethylmaleimide | 22% inhibition at 1 mM | Raphanus sativus | |
| Phenylmethylsulfonylfluoride | 41% inhibition at 1 mM | Raphanus sativus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.51 | - |
L-cysteinylglycine | - |
Raphanus sativus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | addition of Co2+ restores activity after partially inhibition with EDTA | Raphanus sativus | |
| Mn2+ | addition of Mn2+ restores activity partially after inhibition EDTA | Raphanus sativus | |
| Zn2+ | addition of Zn2+ restores activity after partially inhibition with EDTA | Raphanus sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53333 | - |
6 * 53333, gel filtration | Raphanus sativus |
| 55000 | - |
6 * 55000, SDS-PAGE | Raphanus sativus |
| 320000 | - |
gel filtration | Raphanus sativus |
| 330000 | - |
SDS-PAGE | Raphanus sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Raphanus sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G-25 gel filtration, Toyopearl Butyl-650M column chromatography, Toyopearl DEAE-650M column chromatography, and Sephacryl S-200 gel filtration | Raphanus sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Raphanus sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.44 | - |
purified enzyme | Raphanus sativus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10% (v/v) glycerol, few days, activity is greatly diminished | Raphanus sativus |
| -80°C, 10% (v/v) glycerol, few days, activity is greatly diminished | Raphanus sativus |
| 4°C, 10% (v/v) glycerol, few days, remains stable | Raphanus sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gamma-L-glutamyl-L-cysteine + H2O | - |
Raphanus sativus | ? | - |
? | |
| L-cysteinylglycine + H2O | - |
Raphanus sativus | ? | - |
? | |
| additional information | does not hydrolyze gamma-L-glutamyl-p-nitroanilide | Raphanus sativus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 55000, SDS-PAGE | Raphanus sativus |
| homohexamer | 6 * 53333, gel filtration | Raphanus sativus |
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