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Literature summary for 3.4.13.19 extracted from

  • Keynan, S.; Hooper, N.M.; Turner, A.J.
    Identification by site-directed mutagenesis of three essential histidine residues in membrane dipeptidase, a novel mammalian zinc peptidase (1997), Biochem. J., 326, 47-51.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.13.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Cos-1 cells Sus scrofa

Protein Variants

Protein Variants Comment Organism
H128L mutant enzyme exhibits a specific activity and Km for Gly-D-Phe comparable with that of the wild-type enzyme Sus scrofa
H152L inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type, the mutant enzyme fails to bind to cilastatin-Sepharose in contrast to the wild-type enzyme Sus scrofa
H198K inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type Sus scrofa
H20L inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type Sus scrofa
H49K mutant enzyme exhibits a specific activity and Km for Gly-D-Phe comparable with that of the wild-type enzyme Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Cilastatin
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.25
-
 Gly-D-Phe mutant enzyme H128L Sus scrofa
7.1
-
 Gly-D-Phe wild-type enzyme Sus scrofa
12.5
-
 Gly-D-Phe mutant enzyme H49K Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane plasma membrane Sus scrofa 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
leukotriene D4 + H2O Sus scrofa the enzyme is involved in the reanal metabolism of glutathione and its conjugates such as leukotriene ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-D-Phe + H2O
-
 Sus scrofa Gly + D-Phe
-
 ?
leukotriene D4 + H2O the enzyme is involved in the reanal metabolism of glutathione and its conjugates such as leukotriene Sus scrofa ?
-
 ?