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Literature summary for 3.4.13.18 extracted from

  • Uramatsu, M.; Liu, G.; Uramatsu, S.; Zhang, M.; Wang, W.; Nakayama, K.; Manabe, M.; Kodama, H.
    Different effects of sulfur amino acids on prolidase and prolinase activity in normal and prolidase-deficient human erythrocytes (2007), Clin. Chim. Acta, 375, 129-135.
    View publication on PubMed
Search for more literature for 3.4.13.18

Activating Compound

Activating Compound Comment Organism Structure
D-methionine enhances activity at 0-20 mM Homo sapiens
glycine
-
 Homo sapiens
L-alanine
-
 Homo sapiens
Mn2+
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
DL-homocysteine strong inhibition at 10 and 50 mM, in the presence of 0.1 mM MnCl2 Homo sapiens
DL-methionine strong inhibition at 10 and 50 mM, in the presence of 0.1 mM MnCl2 Homo sapiens
L-methionine inhibition at 10 and 50 mM, in the presence of 0.1 mM MnCl2 Homo sapiens
N-acetyl-L-methionine strong inhibition at 10 and 50 mM, in the presence of 0.1 mM MnCl2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.81
-
 Pro-Met in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C Homo sapiens
13.2
-
 Pro-Gly in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C Homo sapiens
13.2
-
 Pro-Gly in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM L-methionine Homo sapiens
13.24
-
 Pro-Met in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM L-methionine Homo sapiens
15.58
-
 Pro-Met in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM L-methionine Homo sapiens
15.85
-
 Pro-Gly in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM L-methionine Homo sapiens
19.1
-
 Pro-Val in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C Homo sapiens
19.54
-
 Pro-Val in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM L-methionine Homo sapiens
22.3
-
 Pro-Met in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM D,L-homocysteine Homo sapiens
23.57
-
 Pro-Gly in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM D,L-homocysteine Homo sapiens
24.69
-
 Pro-Val in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM L-methionine Homo sapiens
45.6
-
 Pro-Val in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 10 mM D,L-homocysteine Homo sapiens
46.78
-
 Pro-Met in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM D,L-homocysteine Homo sapiens
56.33
-
 Pro-Gly in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM D,L-homocysteine Homo sapiens
75.2
-
 Pro-Val in 50 mM Tris-HCl buffer (pH 7.8) with 2 mM or 20 mM MnCl2, at 37°C, in the presence of 50 mM D,L-homocysteine Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Pro-Gly + H2O
-
 Homo sapiens Pro + Gly
-
 ?
Pro-Met + H2O
-
 Homo sapiens Pro + Met
-
 ?
Pro-Val + H2O
-
 Homo sapiens Pro + Val
-
 ?

Synonyms

Synonyms Comment Organism
EC 3.4.13.8 formerly Homo sapiens
prolinase
-
 Homo sapiens