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Literature summary for 3.4.13.18 extracted from

  • Das, M.; Radhakrishnan, A.N.
    Glycyl-L-leucine hydrolase, a versatile master dipeptidase from monkey small intestine (1973), Biochem. J., 135, 609-615.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
Ala-Leu Tris buffer Platyrrhini
1
-
Ala-Leu phosphate buffer Platyrrhini
2.4
-
Gly-Phe Tris buffer Platyrrhini
2.74
-
Gly-Leu Tris buffer Platyrrhini
3.6
-
Gly-Val Tris buffer Platyrrhini
4.4
-
Gly-Ile Tris buffer Platyrrhini
8
-
Gly-Val phosphate buffer Platyrrhini
10
-
Gly-Phe phosphate buffer Platyrrhini
10
-
Gly-Leu phosphate buffer Platyrrhini
10
-
Gly-Ile phosphate buffer Platyrrhini

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Platyrrhini
107000
-
gel filtration Platyrrhini

Organism

Organism UniProt Comment Textmining
Platyrrhini
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
Platyrrhini
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Leu + H2O
-
Platyrrhini Ala + Leu
-
?
Gly-Ile + H2O
-
Platyrrhini Gly + Ile
-
?
Gly-Leu + H2O
-
Platyrrhini Gly + Leu
-
?
Gly-Phe + H2O
-
Platyrrhini Gly + Phe
-
?
Gly-Val + H2O
-
Platyrrhini Gly + Val
-
?
additional information specificity overview Platyrrhini ?
-
?
additional information not: L-Pro-Gly Platyrrhini ?
-
?
additional information not: Gly-Gly Platyrrhini ?
-
?
additional information not: some of the arginine- and aspartic acid-containing dipeptides Platyrrhini ?
-
?
additional information not: Gly-Pro Platyrrhini ?
-
?
additional information not: Gly-L-His Platyrrhini ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Platyrrhini

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1190
-
Ala-Leu phosphate buffer Platyrrhini
6510
-
Ala-Leu Tris buffer Platyrrhini
7450
-
Gly-Val phosphate buffer Platyrrhini
8190
-
Gly-Phe phosphate buffer Platyrrhini
10700
-
Gly-Ile phosphate buffer Platyrrhini
10900
-
Gly-Leu phosphate buffer Platyrrhini
20400
-
Gly-Phe phosphate buffer Platyrrhini
21400
-
Gly-Val Tris buffer Platyrrhini
23000
-
Gly-Ile Tris buffer Platyrrhini
23100
-
Gly-Leu Tris buffer Platyrrhini