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Literature summary for 3.4.13.18 extracted from

  • Patterson, E.K.; Gatmaitan, J.S.; Hayman, S.
    Substrate specificity and pH dependence of dipeptidases purified from Escherichia coli B and from mouse ascites tumor cells (1973), Biochemistry, 12, 3701-3709.
    View publication on PubMed
Search for more literature for 3.4.13.18

General Stability

General Stability Organism
stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 Mus musculus
stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Escherichia coli
2-mercaptoethanol
-
 Mus musculus
cysteine
-
 Escherichia coli
cysteine
-
 Mus musculus
D-Leu-Gly competitive inhibitor of L-Ala-Gly hydrolysis Escherichia coli
D-Leu-Gly competitive inhibitor of L-Ala-Gly hydrolysis Mus musculus
DTT
-
 Escherichia coli
DTT
-
 Mus musculus
iodoacetate not Escherichia coli
iodoacetate not Mus musculus
Iodosobenzoate
-
 Escherichia coli
Iodosobenzoate
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 L-Leu-L-Phe
-
 Escherichia coli
0.25
-
 Leu-Leu tumor enzyme Mus musculus
0.25
-
 Leu-Leu tumor enzyme Escherichia coli
0.28
-
 Ala-Leu tumor enzyme Mus musculus
0.29
-
 Met-Leu
-
 Escherichia coli
0.33
-
 Leu-Gly tumor enzyme Mus musculus
2
-
 Gly-L-Lys
-
 Escherichia coli
29
-
 Gly-L-Asn tumor enzyme Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 gel filtration Mus musculus
100000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
ascites tumor cell
-
 Mus musculus
-

Storage Stability

Storage Stability Organism
-30°C, 0.02 M potassium phosphate, pH 8.2, 0.25 M sucrose, stable Mus musculus
-30°C, 0.02 M potassium phosphate, pH 8.2, 0.25 M sucrose, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ile + H2O best substrate for the tumor enzyme Mus musculus Ala + Ile
-
 ?
Ala-Leu + H2O
-
 Mus musculus Ala + Leu
-
 ?
Gly-L-Asn + H2O
-
 Mus musculus Gly + L-Asn
-
 ?
Gly-L-Lys + H2O
-
 Escherichia coli Gly + L-Lys
-
 ?
L-Ala-Gly + H2O
-
 Mus musculus L-Ala + Gly
-
 ?
L-Ala-Gly + H2O
-
 Escherichia coli L-Ala + Gly
-
 ?
L-Leu-L-Phe + H2O
-
 Escherichia coli L-Leu + L-Phe
-
 ?
Leu-Gly + H2O
-
 Mus musculus Leu + Gly
-
 ?
Leu-Gly + H2O
-
 Escherichia coli Leu + Gly
-
 ?
Leu-Leu + H2O
-
 Mus musculus Leu + Leu
-
 ?
Leu-Leu + H2O
-
 Escherichia coli Leu + Leu
-
 ?
Met-Ala + H2O best substrate for E. coli enzyme Escherichia coli Met + Ala
-
 ?
Met-Leu + H2O
-
 Escherichia coli Met + Leu
-
 ?
additional information specificity overview Mus musculus ?
-
 ?
additional information specificity overview Escherichia coli ?
-
 ?
additional information the tumor enzyme has the highest maximal velocities with substrates containing uncharged bulky C-terminal and small, uncharged N-terminal side chains, the bacterial enzyme hydrolyzes most rapidly substrates with long or flat N-terminal R-groups and small or positively charged C-terminal groups Mus musculus ?
-
 ?
additional information the tumor enzyme has the highest maximal velocities with substrates containing uncharged bulky C-terminal and small, uncharged N-terminal side chains, the bacterial enzyme hydrolyzes most rapidly substrates with long or flat N-terminal R-groups and small or positively charged C-terminal groups Escherichia coli ?
-
 ?
additional information only unsubstituted L-alpha-dipeptides Mus musculus ?
-
 ?
additional information only unsubstituted L-alpha-dipeptides Escherichia coli ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Escherichia coli
8.3
-
 ascites tumor enzyme Mus musculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 6 h, 80% loss of activity, ascites tumor enzyme Mus musculus
6.2 9 6 h stable Escherichia coli
6.7
-
 6 h, 80% loss of activity, mouse ascites tumor enzyme Mus musculus
7
-
 6 h, 25-30% loss of activity, mouse ascites tumor enzyme Mus musculus
8.1 8.3 6 h, stable Mus musculus
8.9
-
 6 h, 25-30% loss of activity, mouse ascites tumor enzyme Mus musculus