General Stability | Organism |
---|---|
stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 | Mus musculus |
stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Escherichia coli | |
2-mercaptoethanol | - |
Mus musculus | |
cysteine | - |
Escherichia coli | |
cysteine | - |
Mus musculus | |
D-Leu-Gly | competitive inhibitor of L-Ala-Gly hydrolysis | Escherichia coli | |
D-Leu-Gly | competitive inhibitor of L-Ala-Gly hydrolysis | Mus musculus | |
DTT | - |
Escherichia coli | |
DTT | - |
Mus musculus | |
iodoacetate | not | Escherichia coli | |
iodoacetate | not | Mus musculus | |
Iodosobenzoate | - |
Escherichia coli | |
Iodosobenzoate | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
L-Leu-L-Phe | - |
Escherichia coli | |
0.25 | - |
Leu-Leu | tumor enzyme | Mus musculus | |
0.25 | - |
Leu-Leu | tumor enzyme | Escherichia coli | |
0.28 | - |
Ala-Leu | tumor enzyme | Mus musculus | |
0.29 | - |
Met-Leu | - |
Escherichia coli | |
0.33 | - |
Leu-Gly | tumor enzyme | Mus musculus | |
2 | - |
Gly-L-Lys | - |
Escherichia coli | |
29 | - |
Gly-L-Asn | tumor enzyme | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
gel filtration | Mus musculus |
100000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ascites tumor cell | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
-30°C, 0.02 M potassium phosphate, pH 8.2, 0.25 M sucrose, stable | Mus musculus |
-30°C, 0.02 M potassium phosphate, pH 8.2, 0.25 M sucrose, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ile + H2O | best substrate for the tumor enzyme | Mus musculus | Ala + Ile | - |
? | |
Ala-Leu + H2O | - |
Mus musculus | Ala + Leu | - |
? | |
Gly-L-Asn + H2O | - |
Mus musculus | Gly + L-Asn | - |
? | |
Gly-L-Lys + H2O | - |
Escherichia coli | Gly + L-Lys | - |
? | |
L-Ala-Gly + H2O | - |
Mus musculus | L-Ala + Gly | - |
? | |
L-Ala-Gly + H2O | - |
Escherichia coli | L-Ala + Gly | - |
? | |
L-Leu-L-Phe + H2O | - |
Escherichia coli | L-Leu + L-Phe | - |
? | |
Leu-Gly + H2O | - |
Mus musculus | Leu + Gly | - |
? | |
Leu-Gly + H2O | - |
Escherichia coli | Leu + Gly | - |
? | |
Leu-Leu + H2O | - |
Mus musculus | Leu + Leu | - |
? | |
Leu-Leu + H2O | - |
Escherichia coli | Leu + Leu | - |
? | |
Met-Ala + H2O | best substrate for E. coli enzyme | Escherichia coli | Met + Ala | - |
? | |
Met-Leu + H2O | - |
Escherichia coli | Met + Leu | - |
? | |
additional information | specificity overview | Mus musculus | ? | - |
? | |
additional information | specificity overview | Escherichia coli | ? | - |
? | |
additional information | the tumor enzyme has the highest maximal velocities with substrates containing uncharged bulky C-terminal and small, uncharged N-terminal side chains, the bacterial enzyme hydrolyzes most rapidly substrates with long or flat N-terminal R-groups and small or positively charged C-terminal groups | Mus musculus | ? | - |
? | |
additional information | the tumor enzyme has the highest maximal velocities with substrates containing uncharged bulky C-terminal and small, uncharged N-terminal side chains, the bacterial enzyme hydrolyzes most rapidly substrates with long or flat N-terminal R-groups and small or positively charged C-terminal groups | Escherichia coli | ? | - |
? | |
additional information | only unsubstituted L-alpha-dipeptides | Mus musculus | ? | - |
? | |
additional information | only unsubstituted L-alpha-dipeptides | Escherichia coli | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
8.3 | - |
ascites tumor enzyme | Mus musculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
6 h, 80% loss of activity, ascites tumor enzyme | Mus musculus |
6.2 | 9 | 6 h stable | Escherichia coli |
6.7 | - |
6 h, 80% loss of activity, mouse ascites tumor enzyme | Mus musculus |
7 | - |
6 h, 25-30% loss of activity, mouse ascites tumor enzyme | Mus musculus |
8.1 | 8.3 | 6 h, stable | Mus musculus |
8.9 | - |
6 h, 25-30% loss of activity, mouse ascites tumor enzyme | Mus musculus |