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Literature summary for 3.4.13.12 extracted from
- Purification and properties of dipeptidase M from Escherichia coli B (1973), J. Biol. Chem., 248, 409-416.
General Stability
| General Stability | Organism |
|---|---|
| generally unstable | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | Mn2+ may restore enzyme activity after EDTA treatment | Escherichia coli |  |
| iodoacetate | 100% inhibition at 10 mM, 10 mM mercaptoethanol restores activity | Escherichia coli | |
| p-chloromercuribenzoate | 100% inhibition at 0.1 mM | Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
Met-Val | - |
Escherichia coli | |
| 0.25 | 0.31 | Met-Ala | - |
Escherichia coli | |
| 0.42 | 1.02 | Met-Ile | - |
Escherichia coli | |
| 0.45 | - |
Met-Leu | - |
Escherichia coli | |
| 0.53 | - |
Met-Thr | - |
Escherichia coli | |
| 0.57 | 0.6 | Met-Ser | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | also protects against heat inactivation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000, SDS-PAGE | Escherichia coli |
| 93000 | 95000 | gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Met-amino acid + H2O | Escherichia coli | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1100 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10 mM imidazole buffer, pH 7.2, 2 mg/ml bovine serum albumin, 30 days, 50% loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Met + H2O | - |
Escherichia coli | Ala + Met | - |
ir | |
| Gly-Gly + H2O | - |
Escherichia coli | Gly + Gly | - |
ir | |
| Leu-Ala + H2O | - |
Escherichia coli | Leu + Ala | - |
ir | |
| Met-Ala + H2O | - |
Escherichia coli | Met + Ala | - |
ir | |
| Met-amino acid + H2O | - |
Escherichia coli | ? | - |
? | |
| Met-Gly + H2O | - |
Escherichia coli | Met + Gly | - |
ir | |
| Met-Ile + H2O | - |
Escherichia coli | Met + Ile | - |
ir | |
| Met-Leu + H2O | - |
Escherichia coli | Met + Leu | - |
ir | |
| Met-Ser + H2O | - |
Escherichia coli | Met + Ser | - |
ir | |
| Met-Thr + H2O | - |
Escherichia coli | Met + Thr | - |
ir | |
| Met-Val + H2O | - |
Escherichia coli | Met + Val | - |
? | |
| Ser-Ala + H2O | - |
Escherichia coli | Ser + Ala | - |
ir | |
| Thr-Ala + H2O | - |
Escherichia coli | Thr + Ala | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 47000, SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
below, 30 min, Mn2+ protects against heat inactivation | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 9 | substrate Met-Ala | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | substrate Met-Ala | Escherichia coli |
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