Literature summary for 3.4.11.B9 extracted from

Russo, S.; Baumann, U.
Crystal structure of a dodecameric tetrahedral-shaped aminopeptidase (2004), J. Biol. Chem., 279, 51275-51281 .

Search for more literature for 3.4.11.B9

Cloned(Commentary)

Cloned (Comment)	Organism
the gene encoding FrvX is amplified by PCR from genomic DNA and cloned into a pET-28a vector introducing a N-terminal His6 tag. The protein is expressed in Escherichia coli Rosetta TM (DE3) cells	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
hexagonal crystals of FrvX are obtained after 2 days by the sitting drop vapor diffusion method. The monomer has a typical clan MH fold. The quaternary structure is built by dimers with a length of 100 A that form the edges of the tetrahedron. All 12 active sites are located on the inside of the tetrahedron. Substrate access is granted by pores with a maximal diameter of 10 A, allowing only small peptides and unfolded proteins access to the active site	Pyrococcus horikoshii

Crystallization (Comment)

Organism

hexagonal crystals of FrvX are obtained after 2 days by the sitting drop vapor diffusion method. The monomer has a typical clan MH fold. The quaternary structure is built by dimers with a length of 100 A that form the edges of the tetrahedron. All 12 active sites are located on the inside of the tetrahedron. Substrate access is granted by pores with a maximal diameter of 10 A, allowing only small peptides and unfolded proteins access to the active site

Pyrococcus horikoshii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	dinuclear zinc active center	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O59196	-	-
Pyrococcus horikoshii OT-3	O59196	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.35	-	substrate: L-leucyl 4-nitroanilide, pH 8.0, 90°C	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
Ala-Ala-Phe 4-nitroanilide + H2O	-	Pyrococcus horikoshii	Ala-Ala-Phe + 4-nitroaniline	-	?
L-alanyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-alanine + 4-nitroaniline	-	?
L-alanyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii OT-3	L-alanine + 4-nitroaniline	-	?
L-arginyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-arginine + 4-nitroaniline	-	?
L-arginyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii OT-3	L-arginine + 4-nitroaniline	-	?
L-glutamyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-glutamine + 4-nitroaniline	-	?
L-leucyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-leucine + 4-nitroaniline	-	?
L-leucyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii OT-3	L-leucine + 4-nitroaniline	-	?
L-lysyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-lysine + 4-nitroaniline	-	?
L-lysyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii OT-3	L-lysine + 4-nitroaniline	-	?
L-methionyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-methionine + 4-nitroaniline	-	?
L-methionyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii OT-3	L-methionine + 4-nitroaniline	-	?
L-prolyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-proline + 4-nitroaniline	-	?
L-valyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-valine + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
dodecamer	-	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
FrvX	-	Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
90	-	assay at	Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Pyrococcus horikoshii