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Literature summary for 3.4.11.B4 extracted from

  • Ando, S.; Ishikawa, K.; Ishida, H.; Kawarabayasi, Y.; Kikuchi, H.; Kosugi, Y.
    Thermostable aminopeptidase from Pyrococcus horikoshii (1999), FEBS Lett., 447, 25-28.
    View publication on PubMed
Search for more literature for 3.4.11.B4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.8
-
 Ala-Ala-Ala-Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii
13.2
-
 Ala-Ala-Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii
18.2
-
 Ala-Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii
39.9
-
 Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme is chelated with one calcium ion per monomer of the protein. Removal of Ca2+ by dialysis for 12 h in 50 mM EDTA does not effect the enzyme activity, suggesting that calcium has no effect on the activity. Replacement of calcium with zinc also shows no effect on the activity Pyrococcus horikoshii
Co2+ activity is dependent on Co2+. 6fold increase in activity in presence of 0.5 mM Co2+ Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36900
-
 x * 36900, SDS-PAGE Pyrococcus horikoshii
440000
-
 gel filtration, HPLC Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58255
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala + H2O
-
 Pyrococcus horikoshii L-alanine + L-alanine
-
 ?
Ala-Ala-Ala + H2O the activity (turnover number) with N-acetyl-Ala-Ala-Ala is 260fold lower compared to the activity with Ala-Ala-Ala Pyrococcus horikoshii Ala-Ala + L-alanine
-
 ?
Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii Ala-Ala-Ala + L-alanine
-
 ?
Ala-Ala-Ala-Ala-Ala + H2O
-
 Pyrococcus horikoshii Ala-Ala-Ala-Ala + L-alanine
-
 ?
additional information no activity with Ala-Pro-Ala Pyrococcus horikoshii ?
-
 ?
N-acetyl-Ala-Ala-Ala + H2O the activity (turnover number) with N-acetyl-Ala-Ala-Ala is 260fold lower compared to the activity with Ala-Ala-Ala Pyrococcus horikoshii Ala-Ala + N-acetyl-L-alanine
-
 ?
N-acetyl-L-alanyl 4-nitroanilide + H2O
-
 Pyrococcus horikoshii N-acetyl-L-alanine + 4-nitroaniline
-
 ?
N-acetyl-L-leucyl 4-nitroanilide + H2O
-
 Pyrococcus horikoshii N-acetyl-L-leucine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 36900, SDS-PAGE Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
PH0519
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
85 98 85°C: about 35% of maximal activity, 98°C: about 85% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 2 h, about 20% loss of activity Pyrococcus horikoshii
95
-
 about 15% loss of activity after 1 h, about 70% loss of activity after 2 h Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.022
-
 N-acetyl-L-alanyl 4-nitroanilide pH 7.5, 85°C Pyrococcus horikoshii
0.116
-
 N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 85°C Pyrococcus horikoshii
0.18
-
 Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii
8.71
-
 Ala-Ala-Ala pH 7.5, 85°C Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
7 8.3 pH 6.5: about 20% of maximal activity, pH 7.0: about 90% of maximal activity, pH 8.3: about 50% of maximal activity Pyrococcus horikoshii