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Literature summary for 3.4.11.9 extracted from

  • Yang, M.; Zheng, J.; Jia, H.; Song, M.
    Functional characterization of X-prolyl aminopeptidase from Toxoplasma gondii (2016), Parasitology, 143, 1443-1449 .
    View publication on PubMed
Search for more literature for 3.4.11.9

Cloned(Commentary)

Cloned (Comment) Organism
gene TgApp, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 Toxoplasma gondii

Protein Variants

Protein Variants Comment Organism
additional information deletion of TgAPP gene in the parasite through a CRISPR/Cas9 system Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
 Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Toxoplasma gondii 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required, activates Toxoplasma gondii
additional information activity of rTgAPP is enhanced by the addition of divalent cations Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Toxoplasma gondii X-prolyl aminopeptidase (APP) is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of N-terminal amino acid present adjacent to a penultimate proline residue ?
-
 ?
additional information Toxoplasma gondii RH X-prolyl aminopeptidase (APP) is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of N-terminal amino acid present adjacent to a penultimate proline residue ?
-
 ?
N6-(2-aminobenzoyl)-L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O Toxoplasma gondii APP cleaves the Lys-Pro peptide bond separating the fluorogenic aminobenzoyl residue and the internal quenching residue 4-nitroanilide N6-(2-aminobenzoyl)-L-Lys + Pro-Pro-4-nitroanilide
-
 ?
N6-(2-aminobenzoyl)-L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O Toxoplasma gondii RH APP cleaves the Lys-Pro peptide bond separating the fluorogenic aminobenzoyl residue and the internal quenching residue 4-nitroanilide N6-(2-aminobenzoyl)-L-Lys + Pro-Pro-4-nitroanilide
-
 ?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-
Toxoplasma gondii RH
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information X-prolyl aminopeptidase (APP) is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of N-terminal amino acid present adjacent to a penultimate proline residue Toxoplasma gondii ?
-
 ?
additional information X-prolyl aminopeptidase (APP) is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of N-terminal amino acid present adjacent to a penultimate proline residue Toxoplasma gondii RH ?
-
 ?
N6-(2-aminobenzoyl)-L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O APP cleaves the Lys-Pro peptide bond separating the fluorogenic aminobenzoyl residue and the internal quenching residue 4-nitroanilide Toxoplasma gondii N6-(2-aminobenzoyl)-L-Lys + Pro-Pro-4-nitroanilide
-
 ?
N6-(2-aminobenzoyl)-L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O APP cleaves the Lys-Pro peptide bond separating the fluorogenic aminobenzoyl residue and the internal quenching residue 4-nitroanilide Toxoplasma gondii RH N6-(2-aminobenzoyl)-L-Lys + Pro-Pro-4-nitroanilide
-
 ?

Synonyms

Synonyms Comment Organism
aminopeptidase P
-
 Toxoplasma gondii
TgAPP
-
 Toxoplasma gondii
X-prolyl aminopeptidase
-
 Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Toxoplasma gondii

General Information

General Information Comment Organism
evolution enzyme TgAPP is a member of the M24 prolyl aminopeptidase family Toxoplasma gondii
malfunction deletion of TgAPP gene in the parasite through a CRISPR/Cas9 system results in inhibition of growth indicating the importance of TgAPP Toxoplasma gondii