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Literature summary for 3.4.11.9 extracted from
- Evidence for catalytic roles for Plasmodium falciparum aminopeptidase P in the food vacuole and cytosol (2009), J. Biol. Chem., 284, 24806-24815.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Escherichia coli as a His-tagged fusion protein | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibitory effect at 1 mM | Plasmodium falciparum |  |
| Ni2+ | inhibitory effect at 1 mM | Plasmodium falciparum | |
| Zn2+ | inhibitory effect at 1 mM | Plasmodium falciparum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
bradykinin | pH 7.5, 37°C | Plasmodium falciparum | |
| 0.51 | - |
FPHFD | pH 7.5, 37°C | Plasmodium falciparum | |
| 0.86 | - |
FPHFD | pH 5.5, 37°C | Plasmodium falciparum | |
| 0.97 | - |
bradykinin | pH 6.5, 37°C | Plasmodium falciparum | |
| 1.4 | - |
YPWTQ | pH 7.5, 37°C | Plasmodium falciparum | |
| 1.8 | - |
YPWTQ | pH 5.5, 37°C | Plasmodium falciparum | |
| 6.7 | - |
bradykinin | pH 5.5, 37°C | Plasmodium falciparum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Plasmodium falciparum | 5829 | - |
| food vacuole | - |
Plasmodium falciparum | 20020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Plasmodium falciparum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
SDS-PAGE, mature form | Plasmodium falciparum |
| 90000 | - |
calculated from cDNA, native form | Plasmodium falciparum |
| 157000 | - |
gel filtration | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bradykinin + H2O | Plasmodium falciparum | potential natural substrate, efficiently hydrolyzed by PfAPP | ? | - |
? | |
| FPHFD + H2O | Plasmodium falciparum | globin pentapeptide sequence, potential natural substrate, efficiently hydrolyzed by PfAPP | ? | - |
? | |
| YPWTQ + H2O | Plasmodium falciparum | globin pentapeptide sequence, potential natural substrate, efficiently hydrolyzed by PfAPP | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Ni2+-charged His-Trap column | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bradykinin + H2O | potential natural substrate, efficiently hydrolyzed by PfAPP | Plasmodium falciparum | ? | - |
? | |
| FPHFD + H2O | globin pentapeptide sequence, potential natural substrate, efficiently hydrolyzed by PfAPP | Plasmodium falciparum | ? | - |
? | |
| Nepsilon-(2-aminobenzoyl)-Lys-Pro-Pro-4-nitroanilide + H2O | - |
Plasmodium falciparum | ? | - |
? | |
| YPWTQ + H2O | globin pentapeptide sequence, potential natural substrate, efficiently hydrolyzed by PfAPP | Plasmodium falciparum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 73000, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase P | - |
Plasmodium falciparum |
| hAPP1 | - |
Homo sapiens |
| PfAPP | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.4 | - |
FPHFD | pH 5.5, 37°C | Plasmodium falciparum | |
| 8.6 | - |
FPHFD | pH 7.5, 37°C | Plasmodium falciparum | |
| 12 | - |
YPWTQ | pH 5.5, 37°C | Plasmodium falciparum | |
| 16 | - |
bradykinin | pH 7.5, 37°C | Plasmodium falciparum | |
| 88 | - |
bradykinin | pH 6.5, 37°C | Plasmodium falciparum | |
| 150 | - |
YPWTQ | pH 7.5, 37°C | Plasmodium falciparum | |
| 160 | - |
bradykinin | pH 5.5, 37°C | Plasmodium falciparum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Plasmodium falciparum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | high stability at 37°C | Plasmodium falciparum |
| 5.5 | - |
human cytosolic APP1 is unstable and forms a high molecular weight aggregate at acidic pH | Homo sapiens |
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