Cloned (Comment) | Organism |
---|---|
- |
Thermococcus onnurineus NA1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
L-Met-L-Pro | pH 5.0, 80°C | Thermococcus onnurineus NA1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | or Mn2+, Zn2+, required. Maximal activity at 3 mM | Thermococcus onnurineus NA1 | |
Mn2+ | or Co2+, Zn2+, required. Maximal activity at 20 mM | Thermococcus onnurineus NA1 | |
Zn2+ | or Mn2+, Co2+, required. Maximal activity at 0.4 mM | Thermococcus onnurineus NA1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39714 | - |
x * 39714, calculated | Thermococcus onnurineus NA1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus onnurineus NA1 | Q2QC92 | expressed in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O | - |
Thermococcus onnurineus NA1 | Abz-L-Lys + L-Pro-L-Pro-4-nitroanilide | - |
? | |
L-Met-L-Pro + H2O | - |
Thermococcus onnurineus NA1 | L-Met + L-Pro | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39714, calculated | Thermococcus onnurineus NA1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Thermococcus onnurineus NA1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
less than 10% of maximum activity | Thermococcus onnurineus NA1 |
50 | - |
less than 10% of maximum activity | Thermococcus onnurineus NA1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life above 100 min | Thermococcus onnurineus NA1 |
90 | - |
half-life 90°C | Thermococcus onnurineus NA1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
541 | - |
L-Met-L-Pro | pH 5.0, 80°C | Thermococcus onnurineus NA1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
substrate L-Met-L-Pro | Thermococcus onnurineus NA1 |
6.5 | 7 | substrate Abz-L-Lys-L-Pro-L-Pro-4-nitroanilide | Thermococcus onnurineus NA1 |