Cloned (Comment) | Organism |
---|---|
gene LeAPP1, DNA and amino acid sequence determination and analysis | Solanum lycopersicum |
gene LeAPP2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli in N-terminal fusion with GST, a mutant form lacking 11 amino acids from the N-terminus is not active | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 50% inhibition at 0.036 mM | Solanum lycopersicum | |
2-mercaptoethanol | 50% inhibition at 0.043 mM | Solanum lycopersicum | |
Ca2+ | stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM | Solanum lycopersicum | |
Co2+ | stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM | Solanum lycopersicum | |
Cu2+ | complete inhibition at 4 mM | Solanum lycopersicum | |
diethyldicarbonate | 50% inhibition at 0.011 mM | Solanum lycopersicum | |
EDTA | 50% inhibition at 0.34 m | Solanum lycopersicum | |
NEM | 50% inhibition at 0.079 mM | Solanum lycopersicum | |
Zn2+ | complete inhibition at 4 mM | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Solanum lycopersicum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM | Solanum lycopersicum | |
Co2+ | stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM | Solanum lycopersicum | |
Mg2+ | stimulation of the enzyme | Solanum lycopersicum | |
Mn2+ | stimulation of the enzyme, most effective at 4 mM | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
x * 105000, recombinant GST-fusion protein, SDS-PAGE | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | Q93X45 | gene LeAPP2, isozyme APP2 | - |
Solanum lycopersicum | Q93X46 | gene LeAPP1, isozyme APP1 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme APP2 from Escherichia coli | Solanum lycopersicum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide | isozyme APP-2 shows a preference for Arg-Pro-Pro-, Arg-Pro-Lys-, Pro-Pro-Gly-, -Phe-Gly- in descending order | Solanum lycopersicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Solanum lycopersicum |
Storage Stability | Organism |
---|---|
-20°C to 4°C, 0.5 mg/ml recombinant GST-fusion enzyme, several weeks without significant loss of activity | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | i.e. RPPGFSPFR, rapid hydrolysis of the N-terminal Arg1-Pro2 bond | Solanum lycopersicum | Arg + PPGFSPFR | - |
? | |
additional information | systemin, a peptide hormone-like signaling molecule from tomato plants, is no substrate | Solanum lycopersicum | ? | - |
? | |
Nalpha-(2-aminobenzoyl)-Lys-Pro-Pro 4-nitroanilide + H2O | - |
Solanum lycopersicum | Nalpha-(2-aminobenzoyl)-Lys + Pro-Pro-4-nitroanilide | - |
? | |
PPGFSPFR + H2O | low activity | Solanum lycopersicum | Pro + PGFSPFR | - |
? | |
substance P + H2O | i.e. RPKPQQFFGLM, hydrolysis of the N-terminal Arg1-Pro2 bond | Solanum lycopersicum | Arg + PKPQQFFGLM | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 105000, recombinant GST-fusion protein, SDS-PAGE | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase P | - |
Solanum lycopersicum |
LeAPP1 | - |
Solanum lycopersicum |
LeAPP2 | - |
Solanum lycopersicum |
More | the enzyme belongs to the peptidase family M24 | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Solanum lycopersicum |