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Literature summary for 3.4.11.9 extracted from

  • Kulkarni, G.V.; Deobagkar, D.D.
    A cytosolic form of aminopeptidase P from Drosophila melanogaster: molecular cloning and characterization (2002), J. Biochem., 131, 445-452.
    View publication on PubMed
Search for more literature for 3.4.11.9

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
Co2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
Cu2+ 56% inhibition at 0.1 mM Drosophila melanogaster
EDTA 80% inhibition at 0.1 mM Drosophila melanogaster
Mg2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
Mn2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM with substrates substance P and bradykinin Drosophila melanogaster
Ni2+ complete inhibition at 0.001 mM Drosophila melanogaster
Zn2+ complete inhibition at 0.001 mM Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Drosophila melanogaster 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
Co2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
Mg2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM Drosophila melanogaster
Mn2+ activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM with substrates substance P and bradykinin Drosophila melanogaster
additional information metalloprotease Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 x * 71000, recombinant His-tagged enzyme, SDS-PAGE Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster O96794 cytosolic isoform
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
brain adult fly, high level Drosophila melanogaster
-
gut adult fly Drosophila melanogaster
-
additional information developmentally regulated Drosophila melanogaster
-
ovary adult fly Drosophila melanogaster
-
testis adult fly Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bradykinin + H2O i.e. RPPGFSPFR, hydrolysis of the N-terminal Arg1-Pro2 bond Drosophila melanogaster Arg + des-Arg-bradykinin i.e. PPGFSPFR ?
substance P + H2O i.e. RPKPQQFFGLM, hydrolysis of the N-terminal Arg1-Pro2 bond Drosophila melanogaster Arg + des-Arg-substance P i.e. PKPQQFFGLM ?

Subunits

Subunits Comment Organism
? x * 71000, recombinant His-tagged enzyme, SDS-PAGE Drosophila melanogaster

Synonyms

Synonyms Comment Organism
aminopeptidase P
-
 Drosophila melanogaster
DAP-P
-
 Drosophila melanogaster
More the enzyme belongs to the peptidase family M24 Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 with substrate substance P Drosophila melanogaster

pH Range

pH Minimum pH Maximum Comment Organism
7.2 8
-
 Drosophila melanogaster