Cloned (Comment) | Organism |
---|---|
transient expression of wild-type and mutants in COS-1 cells | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
D449A | site-directed mutagenesis, inactive mutant | Sus scrofa |
D449N | site-directed mutagenesis, inactive mutant | Sus scrofa |
D460A | site-directed mutagenesis, inactive mutant | Sus scrofa |
D460N | site-directed mutagenesis, inactive mutant | Sus scrofa |
E554A | site-directed mutagenesis, inactive mutant | Sus scrofa |
E554Q | site-directed mutagenesis, inactive mutant | Sus scrofa |
E568A | site-directed mutagenesis, inactive mutant | Sus scrofa |
E568Q | site-directed mutagenesis, low expression level in COS-1 cells, inactive mutant | Sus scrofa |
E588A | site-directed mutagenesis, 66% remaining activity compared to the wild-type enzyme with substrate bradykinin | Sus scrofa |
E588Q | site-directed mutagenesis, 83% remaining activity compared to the wild-type enzyme with substrate bradykinin | Sus scrofa |
H429K | site-directed mutagenesis, inactive mutant | Sus scrofa |
H429L | site-directed mutagenesis, inactive mutant | Sus scrofa |
H519K | site-directed mutagenesis, 2.3% remaining activity compared to the wild-type enzyme with substrate bradykinin, increased Km, reduced kcat | Sus scrofa |
H519L | site-directed mutagenesis, 73.8% remaining activity compared to the wild-type enzyme with substrate bradykinin, increased Km | Sus scrofa |
H523K | site-directed mutagenesis, inactive mutant | Sus scrofa |
H523L | site-directed mutagenesis, inactive mutant | Sus scrofa |
H532K | site-directed mutagenesis, inactive mutant | Sus scrofa |
H532L | site-directed mutagenesis, very low expression level in COS-1 cells, protein degradation, inactive mutant | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
bradykinin | 37°C, wild-type enzyme | Sus scrofa | |
0.391 | - |
bradykinin | 37°C, mutant H519L | Sus scrofa | |
0.5 | - |
bradykinin | 37°C, mutant H519K | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Sus scrofa | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | mono-zinc-containing enzyme, lacks any of the typical metal binding motifs found in other zinc metalloproteases | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
x * 91000, wild-type enzyme, SDS-PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide | acive site configuration, modeling, Asp449, Asp460, His523, Glu554, and Glu568 are in volved in metal binding in the active site, His429 and His523 are involved in shuttling protons during catalysis | Sus scrofa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0375 | - |
recombinant wild-type enzyme, substrate bradykinin | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | hydrolysis of the N-terminal Arg1-Pro2 bond | Sus scrofa | Arg + des-Arg-bradykinin | - |
? | |
Gly-Pro-hydroxyPro + H2O | - |
Sus scrofa | Gly + Pro-hydroxyPro | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 91000, wild-type enzyme, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase P | - |
Sus scrofa |
AP-P | - |
Sus scrofa |
More | the enzyme belongs to the peptidase family M24 | Sus scrofa |
X-Pro aminopeptidase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
bradykinin | 37°C, mutant H519K | Sus scrofa | |
1.18 | - |
bradykinin | 37°C, wild-type enzyme | Sus scrofa | |
1.82 | - |
bradykinin | 37°C, mutant H519L | Sus scrofa | |
2.94 | - |
bradykinin | 37°C, mutant H519L | Sus scrofa | |
6.08 | - |
bradykinin | 37°C, wild-type enzyme | Sus scrofa |