Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.9 extracted from

  • Yoshimoto, T.; Murayama, N.; Honda, T.; Tone, H.; Tsuru, D.
    Cloning and expression of aminopeptidase P gene from Escherichia coli HB101 and characterization of expressed enzyme (1988), J. Biochem., 104, 93-97.
    View publication on PubMed
Search for more literature for 3.4.11.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
 Escherichia coli
CH3HgCl
-
 Escherichia coli
EDTA
-
 Escherichia coli
PCMB partial Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.005 mM, 3fold activation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, SDS-PAGE Escherichia coli
200000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 HB101
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.45
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Pro-p-nitroanilide + H2O
-
 Escherichia coli Ala + Pro-p-nitroanilide
-
 ?
Glu-Pro-p-nitroanilide + H2O
-
 Escherichia coli Glu + Pro-p-nitroanilide
-
 ?
Gly-Pro-2-naphthylamide + H2O
-
 Escherichia coli Gly + Pro-2-naphthylamide
-
 ?
His-Pro-p-nitroanilide + H2O
-
 Escherichia coli His + Pro-p-nitroanilide
-
 ?
Leu-Pro + H2O
-
 Escherichia coli Leu + Pro
-
 ?
Lys-Pro-p-nitroanilide + H2O
-
 Escherichia coli Lys + Pro-p-nitroanilide
-
 ?
Met-Pro + H2O
-
 Escherichia coli Met + Pro
-
 ?
Phe-Pro + H2O
-
 Escherichia coli Phe + Pro
-
 ?
Pro-Pro-Gly-(Pro-Pro-Gly)4 + H2O
-
 Escherichia coli ?
-
 ?
Ser-Pro-p-nitroanilide + H2O
-
 Escherichia coli Ser + Pro-p-nitroanilide
-
 ?
Val-Pro + H2O
-
 Escherichia coli Val + Pro
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 8.0, 30 min, 50% loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 50°C, 30 min, 50% loss of activity Escherichia coli
8 9 stable Escherichia coli