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Literature summary for 3.4.11.7 extracted from

  • Robinson, M.; Buchtmann, K.; Jenkins, C.; Tacchi, J.; Raymond, B.; To, J.; Chowdhury, P.; Woolley, L.; Labbate, M.; Turnbull, L.; Whitchurch, C.; Padula, M.; Djordjevic, S.
    MHJ-0125 is an M42 glutamyl aminopeptidase that moonlights as a multifunctional adhesin on the surface of Mycoplasma hyopneumoniae (2013), Open Biology, 3, 130017.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.7

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycoplasma hyopneumoniae

Inhibitors

Inhibitors Comment Organism Structure
bestatin 1 mM, 13% residual activity Mycoplasma hyopneumoniae
Cu2+ 5 mM, 3.5% residual activiy Mycoplasma hyopneumoniae
EDTA 10 mM, 3.7% residual activiy Mycoplasma hyopneumoniae
Mn2+ 5 mM, 15% residual activiy Mycoplasma hyopneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface enzyme resides in the cytosol, but a subset of molecules are both bound to and secreted from the cell surface Mycoplasma hyopneumoniae 9986
-
cytosol enzyme resides in the cytosol, but a subset of molecules are both bound to and secreted from the cell surface Mycoplasma hyopneumoniae 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, 52% activation Mycoplasma hyopneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39200
-
 12 * 39200, calculated, 12 * 40000, SDS-PAGE Mycoplasma hyopneumoniae
40000
-
 12 * 39200, calculated, 12 * 40000, SDS-PAGE Mycoplasma hyopneumoniae
440000
-
 gel filtration Mycoplasma hyopneumoniae
600000
-
 PAGE Mycoplasma hyopneumoniae

Organism

Organism UniProt Comment Textmining
Mycoplasma hyopneumoniae Q4AAK4
-
-
Mycoplasma hyopneumoniae ATCC 25934 Q4AAK4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-methylcoumaryl-7-amide + H2O about 50% of the activity with L-Glu-4-methylcoumaryl-7-amide Mycoplasma hyopneumoniae L-Ala + 7-amino-4-methylcoumarin
-
 ?
L-Ala-4-methylcoumaryl-7-amide + H2O about 50% of the activity with L-Glu-4-methylcoumaryl-7-amide Mycoplasma hyopneumoniae ATCC 25934 L-Ala + 7-amino-4-methylcoumarin
-
 ?
L-Glu-4-methylcoumaryl-7-amide + H2O
-
 Mycoplasma hyopneumoniae L-Glu + 7-amino-4-methylcoumarin
-
 ?
L-Glu-4-methylcoumaryl-7-amide + H2O
-
 Mycoplasma hyopneumoniae ATCC 25934 L-Glu + 7-amino-4-methylcoumarin
-
 ?
L-Leu-4-methylcoumaryl-7-amide + H2O
-
 Mycoplasma hyopneumoniae L-Leu + 7-amino-4-methylcoumarin about 20% of the activity with L-Glu-4-methylcoumaryl-7-amide ?
L-Leu-4-methylcoumaryl-7-amide + H2O
-
 Mycoplasma hyopneumoniae ATCC 25934 L-Leu + 7-amino-4-methylcoumarin about 20% of the activity with L-Glu-4-methylcoumaryl-7-amide ?

Subunits

Subunits Comment Organism
dodecamer 12 * 39200, calculated, 12 * 40000, SDS-PAGE Mycoplasma hyopneumoniae

Synonyms

Synonyms Comment Organism
MHJ_0125
-
 Mycoplasma hyopneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Mycoplasma hyopneumoniae

pI Value

Organism Comment pI Value Maximum pI Value
Mycoplasma hyopneumoniae calculated
-
 6.2

General Information

General Information Comment Organism
physiological function enzyme acts as a multifunctional adhesin, binding to both heparin and plasminogen. It binds plasminogen and facilitates its activation by tissue plasminogen activator. Plasmin cleaves host extracellular matrix proteins and activates matrix metalloproteases, generating peptide substrates for the enzyme and a source of amino acids for growth of Mycoplasma hyopneumoniae Mycoplasma hyopneumoniae