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Literature summary for 3.4.11.7 extracted from

  • Claperon, C.; Rozenfeld, R.; Iturrioz, X.; Inguimbert, N.; Okada, M.; Roques, B.; Maigret, B.; Llorens-Cortes, C.
    Asp218 participates with Asp213 to bind a Ca2+ atom into the S1 subsite of aminopeptidase A: a key element for substrate specificity (2008), Biochem. J., 416, 37-46.
    View publication on PubMed
Search for more literature for 3.4.11.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in CHO-K1 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information substitution mutations at Asp218 and Asp213 maintain the ability of APA to be activated by Ca2+ and induce a decrease of the inhibitory potencies of inhibitors homologous with acidic residues Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
glutamate phosphonate specific aminopeptidase A inhibitor Mus musculus
N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.152
-
 L-Glu-p-nitroanilide wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C Mus musculus
1.475
-
 L-Glu-p-nitroanilide wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ each APA monomer contains one Ca2+ atom, Ca2+ (from 0 to 4 mM) increases the activity of the wild type enzyme Mus musculus
Zn2+ contains zinc Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 minor immature form, SDS-PAGE Mus musculus
160000
-
 major mature form, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P16406
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon Co2+ column chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin II + H2O
-
 Mus musculus aspartic acid + angiotensin III
-
 ?
L-Glu-p-nitroanilide + H2O
-
 Mus musculus L-Glu + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
aminopeptidase A
-
 Mus musculus
APA
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
295
-
 L-Glu-p-nitroanilide wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C Mus musculus
720
-
 L-Glu-p-nitroanilide wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00007
-
 N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide wild type enzyme, in 50 mM Tris/HCl buffer (pH 7.4), 4 mM Ca2+, at 37°C Mus musculus