Cloned (Comment) | Organism |
---|---|
into the pENTR-D-TOPO vector and into the baculovirus transfer vector pDEST8 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D221A | mutant constructed for the examination of Ca2+ effects on enzymatic activity | Homo sapiens |
D221E | mutant constructed for the examination of Ca2+ effects on enzymatic activity | Homo sapiens |
D221N | mutant constructed for the examination of Ca2+ effects on enzymatic activity | Homo sapiens |
D221Q | mutant constructed for the examination of Ca2+ effects on enzymatic activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | - |
Homo sapiens | |
angiotensin IV | - |
Homo sapiens | |
Zn2+ | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
kallidin | wild-type, in the absence of CaCl2 | Homo sapiens | |
0.37 | - |
kallidin | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
0.5 | - |
Glu-7-amido-4-methylcoumarin | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
0.6 | - |
kallidin | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
0.84 | - |
angiotensin II | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
1.13 | - |
kallidin | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
1.18 | - |
angiotensin II | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
1.21 | - |
cholecystokinin-8 | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
1.22 | - |
angiotensin II | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
1.54 | - |
Glu-7-amido-4-methylcoumarin | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
1.65 | - |
Glu-7-amido-4-methylcoumarin | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
1.67 | - |
Glu-7-amido-4-methylcoumarin | mutant D221Q, in the absence of CaCl2 | Homo sapiens | |
1.84 | - |
Glu-7-amido-4-methylcoumarin | wild-type, in the absence of CaCl2 | Homo sapiens | |
1.84 | - |
neurokinin B | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
1.95 | - |
Glu-7-amido-4-methylcoumarin | mutant D221Q, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
2.85 | - |
angiotensin II | wild-type, in the absence of CaCl2 | Homo sapiens | |
2.87 | - |
Glu-7-amido-4-methylcoumarin | mutant D221E, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
2.9 | - |
cholecystokinin-8 | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
2.93 | - |
cholecystokinin-8 | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
3.25 | - |
cholecystokinin-8 | wild-type, in the absence of CaCl2 | Homo sapiens | |
3.61 | - |
Glu-7-amido-4-methylcoumarin | mutant D221E, in the absence of CaCl2 | Homo sapiens | |
3.97 | - |
neurokinin B | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
4.3 | - |
neurokinin B | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
4.31 | - |
Glu-7-amido-4-methylcoumarin | mutant D221A, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
4.36 | - |
Glu-7-amido-4-methylcoumarin | mutant D221A, in the absence of CaCl2 | Homo sapiens | |
4.94 | - |
neurokinin B | wild-type, in the absence of CaCl2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin II + H2O | Homo sapiens | angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe | aspartic acid + angiotensin III | angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Co2+-charged chelating Toyopearl column | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin II + H2O | angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe | Homo sapiens | aspartic acid + angiotensin III | angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe | ? | |
cholecystokinin-8 + H2O | CCK8 | Homo sapiens | ? | - |
? | |
Glu-7-amido-4-methylcoumarin + H2O | activity assay | Homo sapiens | Glu + 7-amino-4-methylcoumarin | - |
? | |
kallidin + H2O | - |
Homo sapiens | ? | - |
? | |
neurokinin B + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase A | - |
Homo sapiens |
hAPA | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | 0.51 | cholecystokinin-8 | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
0.031 | 0.51 | cholecystokinin-8 | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
0.052 | 2.1 | neurokinin B | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
0.052 | 2.1 | cholecystokinin-8 | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
0.58 | - |
Glu-7-amido-4-methylcoumarin | mutant D221A, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
0.7 | - |
kallidin | wild-type, in the absence of CaCl2 | Homo sapiens | |
0.72 | - |
Glu-7-amido-4-methylcoumarin | mutant D221A, in the absence of CaCl2 | Homo sapiens | |
0.79 | - |
kallidin | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
0.88 | - |
Glu-7-amido-4-methylcoumarin | mutant D221E, in the absence of CaCl2 | Homo sapiens | |
1.23 | - |
Glu-7-amido-4-methylcoumarin | mutant D221E, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
1.33 | - |
kallidin | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
1.55 | - |
kallidin | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
2.76 | - |
angiotensin II | wild-type, in the absence of CaCl2 | Homo sapiens | |
2.78 | - |
Glu-7-amido-4-methylcoumarin | mutant D221Q, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
2.96 | - |
Glu-7-amido-4-methylcoumarin | mutant D221Q, in the absence of CaCl2 | Homo sapiens | |
3 | 6 | angiotensin II | wild-type, in the absence of CaCl2 | Homo sapiens | |
3.01 | - |
angiotensin II | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
3.22 | - |
angiotensin II | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
3.4 | - |
Glu-7-amido-4-methylcoumarin | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
3.41 | - |
Glu-7-amido-4-methylcoumarin | wild-type, in the absence of CaCl2 | Homo sapiens | |
3.68 | - |
Glu-7-amido-4-methylcoumarin | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
3.88 | - |
angiotensin II | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
5.25 | - |
Glu-7-amido-4-methylcoumarin | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
7.71 | - |
cholecystokinin-8 | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
7.81 | - |
neurokinin B | wild-type, in the absence of CaCl2 | Homo sapiens | |
8.22 | - |
cholecystokinin-8 | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
8.87 | - |
neurokinin B | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
9.18 | - |
cholecystokinin-8 | wild-type, in the absence of CaCl2 | Homo sapiens | |
9.8 | - |
neurokinin B | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
9.95 | - |
cholecystokinin-8 | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
10.47 | - |
neurokinin B | mutant D221N, in the absence of CaCl2 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000059 | - |
amastatin | mutant D221, in the presence of 1 mM CaCl2 | Homo sapiens | |
0.0000177 | - |
amastatin | mutant D221, in the absence of CaCl2 | Homo sapiens | |
0.0000243 | - |
amastatin | wild-type enzyme, in the absence of CaCl2 | Homo sapiens | |
0.0001927 | - |
amastatin | wild-type enzyme, in the presence of 1 mM CaCl2 | Homo sapiens | |
0.00026 | - |
angiotensin IV | mutant D221, in the absence of CaCl2 | Homo sapiens | |
0.000324 | - |
angiotensin IV | mutant D221, in the presence of 1 mM CaCl2 | Homo sapiens | |
0.000386 | - |
angiotensin IV | wild-type enzyme, in the absence of CaCl2 | Homo sapiens | |
0.001297 | - |
angiotensin IV | wild-type enzyme, in the presence of 1 mM CaCl2 | Homo sapiens |