Application | Comment | Organism |
---|---|---|
medicine | enzyme is a promising target for new anti-malarial drug development | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
using codons for optimized gene expression in the yeast Pichia pastoris, for protein expression Sf9 insect cells are infected with PfM18AAP recombinant Baculovirus, furthermore pGEM, pHGFPB and pHcmycB are used | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1-aminopropane-1,3-diyl)bis(phosphonic acid) | - |
Plasmodium falciparum | |
(3R)-3-amino-3-phosphonopropanoic acid | - |
Plasmodium falciparum | |
3-amino-3-[hydroxy(phenyl)phosphoryl]propanoic acid | - |
Plasmodium falciparum | |
3-[(1-amino-2-carboxyethyl)(hydroxy)phosphoryl]-2-methylpropanoic acid | - |
Plasmodium falciparum | |
3-[[amino(carboxy)methyl](hydroxy)phosphoryl]-2-methylpropanoic acid | - |
Plasmodium falciparum | |
5-amino-5-phosphonopentanoic acid | - |
Plasmodium falciparum | |
amino(phosphono)acetic acid | - |
Plasmodium falciparum | |
amino[hydroxy(methyl)phosphoryl]acetic acid | - |
Plasmodium falciparum | |
EDTA | activity is reduced to 14% after incubating with 10 mM EDTA | Plasmodium falciparum | |
o-phenanthroline | activity is reduced to 8.3% after incubating with 20 mM o-phenanthroline | Plasmodium falciparum | |
Zn2+ | Zn2+ abolishes enzyme activity at a concentration of 1 mM | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1351 | - |
Glu-7-amido-4-methylcoumarin | in the absence of cobalt | Plasmodium falciparum | |
0.1363 | - |
Glu-7-amido-4-methylcoumarin | in the presence of 1 mM cobalt | Plasmodium falciparum | |
0.3273 | - |
Asp-7-amido-4-methylcoumarin | in the presence of 1 mM cobalt | Plasmodium falciparum | |
0.3848 | - |
Asp-7-amido-4-methylcoumarin | in the absence of cobalt | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Plasmodium falciparum | 5829 | - |
vacuole | - |
Plasmodium falciparum | 5773 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
determined by reducing SDS-PAGE | Plasmodium falciparum |
560000 | - |
octamer, determined by gel filtration HPLC on a Superdex-200 column | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8I2J3 | - |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography on a Ni-NTA column | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ring stage | - |
Plasmodium falciparum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
607 | - |
Glu-7-amido-4-methylcoumarin | Plasmodium falciparum |
662.5 | - |
Asp-7-amido-4-methylcoumarin | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Asp-7-amido-4-methylcoumarin + H2O | assay for measuring aspartyl aminopeptidase activity | Plasmodium falciparum | Asp + 7-amino-4-methylcoumarin | - |
? | |
Glu-7-amido-4-methylcoumarin + H2O | assay for measuring aspartyl aminopeptidase activity | Plasmodium falciparum | Glu + 7-amino-4-methylcoumarin | - |
? | |
Leu-7-amido-4-methylcoumarin + H2O | assay for measuring aspartyl aminopeptidase activity | Plasmodium falciparum | Leu + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | - |
Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
Aminopeptidase | - |
Plasmodium falciparum |
aspartyl aminopeptidase | - |
Plasmodium falciparum |
M18 aspartyl aminopeptidase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
Glu-7-amido-4-methylcoumarin | in the absence of cobalt | Plasmodium falciparum | |
0.05 | - |
Asp-7-amido-4-methylcoumarin | in the absence of cobalt | Plasmodium falciparum | |
0.33 | - |
Glu-7-amido-4-methylcoumarin | in the presence of 1 mM cobalt | Plasmodium falciparum | |
0.354 | - |
Asp-7-amido-4-methylcoumarin | in the presence of 1 mM cobalt | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
(3R)-3-amino-3-phosphonopropanoic acid | - |
Plasmodium falciparum | |
0.0044 | - |
(1-aminopropane-1,3-diyl)bis(phosphonic acid) | - |
Plasmodium falciparum | |
0.0271 | - |
amino(phosphono)acetic acid | - |
Plasmodium falciparum | |
0.0427 | - |
3-[(1-amino-2-carboxyethyl)(hydroxy)phosphoryl]-2-methylpropanoic acid | - |
Plasmodium falciparum | |
0.105 | - |
5-amino-5-phosphonopentanoic acid | - |
Plasmodium falciparum | |
0.1831 | - |
3-[[amino(carboxy)methyl](hydroxy)phosphoryl]-2-methylpropanoic acid | - |
Plasmodium falciparum | |
0.395 | - |
amino[hydroxy(methyl)phosphoryl]acetic acid | - |
Plasmodium falciparum | |
2 | - |
3-amino-3-[hydroxy(phenyl)phosphoryl]propanoic acid | - |
Plasmodium falciparum |