Protein Variants | Comment | Organism |
---|---|---|
Q169E | mutant shows no activity | Homo sapiens |
Q169N | mutant shows a significant decrease in hydrolytic activity toward the fluorescent substrate Lys-4-methylcoumaryl-7-amide (MCA). Mutant shows an increase in IC50 values of inhibitors that interact with the catalytic pocket of aminopeptidase B. Km (Arg 4-methylcoumarin 7-amide) similar to wild-type, kcat (Arg 4-methylcoumarin 7-amide) decreased compared to wild-type. Km (Lys 4-methylcoumarin 7-amide) increased compared to wild-type, kcat (Lys 4-methylcoumarin 7-amide) decreased compared to wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
arphamenine A | - |
Homo sapiens | |
Arphamenine B | - |
Homo sapiens | |
bestatin | - |
Homo sapiens | |
kallidin | human peptide hormone, 10 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 73.7% (wild-type enzyme) | Homo sapiens | |
Substance P | human peptide hormone, 100 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 63.8% (wild-type enzyme); human peptide hormone, 10 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 78% (wild-type enzyme) | Homo sapiens | |
ZnCl2 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0157 | - |
kallidin | pH 7.4, 37°C, wild-type | Homo sapiens | |
0.021 | - |
Arg-Met-enkephalin | pH 7.4, 37°C, wild-type | Homo sapiens | |
0.0251 | - |
Met-enkephalin | pH 7.4, 37°C, wild-type | Homo sapiens | |
0.0533 | - |
Arg 4-methylcoumarin 7-amide | pH 7.4, 37°C, wild-type | Homo sapiens | |
0.0651 | - |
kallidin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
0.0774 | - |
Arg 4-methylcoumarin 7-amide | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
0.111 | - |
Arg-Met-enkephalin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
0.36 | - |
Met-enkephalin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
0.381 | - |
Lys 4-methylcoumarin 7-amide | pH 7.4, 37°C, wild-type | Homo sapiens | |
0.568 | - |
Lys 4-methylcoumarin 7-amide | pH 7.4, 37°C, mutant Q169N | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg 4-methylcoumarin 7-amide + H2O | - |
Homo sapiens | Arg + 7-amino-4-methylcoumarin | - |
? | |
Arg-Met-enkephalin + H2O | - |
Homo sapiens | ? | - |
? | |
kallidin + H2O | - |
Homo sapiens | ? | - |
? | |
Lys 4-methylcoumarin 7-amide + H2O | - |
Homo sapiens | Lys + 7-amino-4-methylcoumarin | - |
? | |
Met-enkephalin + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase B | - |
Homo sapiens |
APB | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
Met-enkephalin | pH 7.4, 37°C, wild-type | Homo sapiens | |
0.48 | - |
Met-enkephalin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
1.07 | - |
Arg-Met-enkephalin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
1.95 | - |
Lys 4-methylcoumarin 7-amide | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
1.99 | - |
kallidin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
2.35 | - |
kallidin | pH 7.4, 37°C, wild-type | Homo sapiens | |
3.63 | - |
Arg-Met-enkephalin | pH 7.4, 37°C, wild-type | Homo sapiens | |
4.06 | - |
Arg 4-methylcoumarin 7-amide | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
9.27 | - |
Arg 4-methylcoumarin 7-amide | pH 7.4, 37°C, wild-type | Homo sapiens | |
14.9 | - |
Lys 4-methylcoumarin 7-amide | pH 7.4, 37°C, wild-type | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000072 | - |
pH 7.4, 37°C, wild-type | Homo sapiens | Arphamenine B | |
0.00000155 | - |
pH 7.4, 37°C, mutant Q169N | Homo sapiens | Arphamenine B | |
0.00000316 | - |
pH 7.4, 37°C, mutant Q169N | Homo sapiens | arphamenine A | |
0.00000349 | - |
pH 7.4, 37°C, wild-type | Homo sapiens | bestatin | |
0.00000373 | - |
pH 7.4, 37°C, wild-type | Homo sapiens | arphamenine A | |
0.00000391 | - |
pH 7.4, 37°C, wild-type | Homo sapiens | ZnCl2 | |
0.00000592 | - |
pH 7.4, 37°C, mutant Q169N | Homo sapiens | ZnCl2 | |
0.00006 | - |
pH 7.4, 37°C, mutant Q169N | Homo sapiens | 1,10-phenanthroline | |
0.000077 | - |
pH 7.4, 37°C, wild-type | Homo sapiens | 1,10-phenanthroline | |
0.000289 | - |
pH 7.4, 37°C, mutant Q169N | Homo sapiens | bestatin |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
Met-enkephalin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
10.4 | - |
Arg-Met-enkephalin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
19 | - |
Met-enkephalin | pH 7.4, 37°C, wild-type | Homo sapiens | |
30.1 | - |
kallidin | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
156 | - |
kallidin | pH 7.4, 37°C, wild-type | Homo sapiens | |
173 | - |
Arg-Met-enkephalin | pH 7.4, 37°C, wild-type | Homo sapiens | |
3440 | - |
Lys 4-methylcoumarin 7-amide | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
39200 | - |
Lys 4-methylcoumarin 7-amide | pH 7.4, 37°C, wild-type | Homo sapiens | |
52400 | - |
Arg 4-methylcoumarin 7-amide | pH 7.4, 37°C, mutant Q169N | Homo sapiens | |
175000 | - |
Arg 4-methylcoumarin 7-amide | pH 7.4, 37°C, wild-type | Homo sapiens |