Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 0.1 mM | Capnocytophaga granulosa | |
Cu2+ | 84% inhibition at 0.1 mM | Capnocytophaga granulosa | |
Hg2+ | complete inhibition at 0.1 mM | Capnocytophaga granulosa | |
additional information | no inhibition at 0.1 mM by Mg2+ | Capnocytophaga granulosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 26% activation at 0.1 mM | Capnocytophaga granulosa | |
Fe3+ | 29% activation at 0.1 mM | Capnocytophaga granulosa | |
Zn2+ | 34% activation at 0.1 mM | Capnocytophaga granulosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
x * 86000, SDS-PAGE | Capnocytophaga granulosa |
270000 | - |
gel filtration | Capnocytophaga granulosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capnocytophaga granulosa | - |
strain ATCC 51502 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 159000fold by ammonium sulfate fractionation, ultrafiltration, cation exchange chromatography and gel filtration | Capnocytophaga granulosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
297.9 | - |
purified enzyme | Capnocytophaga granulosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-2-naphthylamide + H2O | - |
Capnocytophaga granulosa | L-Ala + 2-naphthylamine | - |
? | |
L-Arg-2-naphthylamide + H2O | - |
Capnocytophaga granulosa | L-Arg + 2-naphthylamine | - |
? | |
L-Leu-2-naphthylamide + H2O | - |
Capnocytophaga granulosa | L-Leu + 2-naphthylamine | - |
? | |
L-Lys-2-naphthylamide + H2O | - |
Capnocytophaga granulosa | L-Lys + 2-naphthylamine | - |
? | |
L-Met-2-naphthylamide + H2O | - |
Capnocytophaga granulosa | L-Met + 2-naphthylamine | - |
? | |
additional information | substrate specificity, no activity with benzoyl-Arg-2-naphthylamide, and 2-naphthylamides of Glu, Ser, and Tyr, no activity with Phe-Phe-Ala-2-naphthylamide, Leu-Gly-Gly-4-methoxy-2-naphthylamide, and Gly-Pro-Leu-2-naphthylamide, overview | Capnocytophaga granulosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 86000, SDS-PAGE | Capnocytophaga granulosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Capnocytophaga granulosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, complete loss of activity | Capnocytophaga granulosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Capnocytophaga granulosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7 | stable | Capnocytophaga granulosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Capnocytophaga granulosa | band I, isoelectric fosusing | - |
7.5 |
Capnocytophaga granulosa | band II, isoelectric fosusing | - |
8.8 |