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Literature summary for 3.4.11.6 extracted from

  • Ohishi, K.; Yamamoto, T.; Tomofuji, T.; Tamaki, N.; Watanabe, T.
    Isolation and characterization of aminopeptidase from Capnocytophaga granulosa ATCC 51502 (2005), Oral Microbiol. Immunol., 20, 67-72.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 0.1 mM Capnocytophaga granulosa
Cu2+ 84% inhibition at 0.1 mM Capnocytophaga granulosa
Hg2+ complete inhibition at 0.1 mM Capnocytophaga granulosa
additional information no inhibition at 0.1 mM by Mg2+ Capnocytophaga granulosa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 26% activation at 0.1 mM Capnocytophaga granulosa
Fe3+ 29% activation at 0.1 mM Capnocytophaga granulosa
Zn2+ 34% activation at 0.1 mM Capnocytophaga granulosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
x * 86000, SDS-PAGE Capnocytophaga granulosa
270000
-
gel filtration Capnocytophaga granulosa

Organism

Organism UniProt Comment Textmining
Capnocytophaga granulosa
-
strain ATCC 51502
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 159000fold by ammonium sulfate fractionation, ultrafiltration, cation exchange chromatography and gel filtration Capnocytophaga granulosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
297.9
-
purified enzyme Capnocytophaga granulosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-2-naphthylamide + H2O
-
Capnocytophaga granulosa L-Ala + 2-naphthylamine
-
?
L-Arg-2-naphthylamide + H2O
-
Capnocytophaga granulosa L-Arg + 2-naphthylamine
-
?
L-Leu-2-naphthylamide + H2O
-
Capnocytophaga granulosa L-Leu + 2-naphthylamine
-
?
L-Lys-2-naphthylamide + H2O
-
Capnocytophaga granulosa L-Lys + 2-naphthylamine
-
?
L-Met-2-naphthylamide + H2O
-
Capnocytophaga granulosa L-Met + 2-naphthylamine
-
?
additional information substrate specificity, no activity with benzoyl-Arg-2-naphthylamide, and 2-naphthylamides of Glu, Ser, and Tyr, no activity with Phe-Phe-Ala-2-naphthylamide, Leu-Gly-Gly-4-methoxy-2-naphthylamide, and Gly-Pro-Leu-2-naphthylamide, overview Capnocytophaga granulosa ?
-
?

Subunits

Subunits Comment Organism
oligomer x * 86000, SDS-PAGE Capnocytophaga granulosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Capnocytophaga granulosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, complete loss of activity Capnocytophaga granulosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Capnocytophaga granulosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.6 7 stable Capnocytophaga granulosa

pI Value

Organism Comment pI Value Maximum pI Value
Capnocytophaga granulosa band I, isoelectric fosusing
-
7.5
Capnocytophaga granulosa band II, isoelectric fosusing
-
8.8