Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition at 1 mM | Toxoplasma gondii | |
DTT | 9% inhibition at 1 mM | Toxoplasma gondii | |
EDTA | 90% inhibition at 1 mM | Toxoplasma gondii | |
HgCl2 | complete inhibition at 1 mM | Toxoplasma gondii | |
iodoacetamide | 16% inhibition at 1 mM | Toxoplasma gondii | |
leupeptin | 5% inhibition at 0.01 mM | Toxoplasma gondii | |
additional information | no inhibition by phosphoramidon, E64, antipaine, and TLCK | Toxoplasma gondii | |
NEM | 13% inhibition at 1 mM | Toxoplasma gondii | |
pepstatin | 5% inhibition at 0.01 mM | Toxoplasma gondii | |
PMSF | 14% inhibition at 1 mM | Toxoplasma gondii | |
ZnCl2 | 96% inhibition at 1 mM | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Toxoplasma gondii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metallopeptidase | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
x * 110000, reducing SDS-PAGE | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
80.9fold to homogeneity by gel filtration, anion exchange chromatography, and again gel filtration | Toxoplasma gondii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
purified enzyme, substrate N-succinyl-Gly-Pro-Leu-Gly-Pro 7-amido-4-methylcoumarin | Toxoplasma gondii |
55 | - |
purified enzyme, substrate L-Leu 7-amido-4-methylcoumarin | Toxoplasma gondii |
140 | - |
purified enzyme, substrate L-Tyr 7-amido-4-methylcoumarin | Toxoplasma gondii |
263.7 | - |
purified enzyme, substrate L-Arg 7-amido-4-methylcoumarin | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg 7-amido-4-methylcoumarin + H2O | fluorogenic substrate, best substrate | Toxoplasma gondii | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Leu 7-amido-4-methylcoumarin + H2O | fluorogenic substrate | Toxoplasma gondii | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
L-Tyr 7-amido-4-methylcoumarin + H2O | fluorogenic substrate | Toxoplasma gondii | L-Tyr + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Gly-Pro-Leu-Gly-Pro 7-amido-4-methylcoumarin + H2O | fluorogenic substrate | Toxoplasma gondii | N-succinyl-Gly-Pro-Leu-Gly-Pro + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, reducing SDS-PAGE | Toxoplasma gondii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
30 min, completely stable | Toxoplasma gondii |
40 | - |
30 min, 95% activity remaining | Toxoplasma gondii |
55 | - |
30 min, 50% activity remaining | Toxoplasma gondii |
60 | - |
30 min, residual activity remaining | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Toxoplasma gondii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH profile | Toxoplasma gondii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Toxoplasma gondii | chromatofocusing | - |
4.7 |