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Literature summary for 3.4.11.6 extracted from

  • Berthonneau, J.; Rodier, M.H.; El Moudni, B.; Jacquemin, J.L.
    Toxoplasma gondii: Purification and characterization of an immunogenic metallopeptidase (2000), Exp. Parasitol., 95, 158-162.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition at 1 mM Toxoplasma gondii
DTT 9% inhibition at 1 mM Toxoplasma gondii
EDTA 90% inhibition at 1 mM Toxoplasma gondii
HgCl2 complete inhibition at 1 mM Toxoplasma gondii
iodoacetamide 16% inhibition at 1 mM Toxoplasma gondii
leupeptin 5% inhibition at 0.01 mM Toxoplasma gondii
additional information no inhibition by phosphoramidon, E64, antipaine, and TLCK Toxoplasma gondii
NEM 13% inhibition at 1 mM Toxoplasma gondii
pepstatin 5% inhibition at 0.01 mM Toxoplasma gondii
PMSF 14% inhibition at 1 mM Toxoplasma gondii
ZnCl2 96% inhibition at 1 mM Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Toxoplasma gondii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metallopeptidase Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
x * 110000, reducing SDS-PAGE Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
80.9fold to homogeneity by gel filtration, anion exchange chromatography, and again gel filtration Toxoplasma gondii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
purified enzyme, substrate N-succinyl-Gly-Pro-Leu-Gly-Pro 7-amido-4-methylcoumarin Toxoplasma gondii
55
-
purified enzyme, substrate L-Leu 7-amido-4-methylcoumarin Toxoplasma gondii
140
-
purified enzyme, substrate L-Tyr 7-amido-4-methylcoumarin Toxoplasma gondii
263.7
-
purified enzyme, substrate L-Arg 7-amido-4-methylcoumarin Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg 7-amido-4-methylcoumarin + H2O fluorogenic substrate, best substrate Toxoplasma gondii L-Arg + 7-amino-4-methylcoumarin
-
?
L-Leu 7-amido-4-methylcoumarin + H2O fluorogenic substrate Toxoplasma gondii L-Leu + 7-amino-4-methylcoumarin
-
?
L-Tyr 7-amido-4-methylcoumarin + H2O fluorogenic substrate Toxoplasma gondii L-Tyr + 7-amino-4-methylcoumarin
-
?
N-succinyl-Gly-Pro-Leu-Gly-Pro 7-amido-4-methylcoumarin + H2O fluorogenic substrate Toxoplasma gondii N-succinyl-Gly-Pro-Leu-Gly-Pro + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
? x * 110000, reducing SDS-PAGE Toxoplasma gondii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
30 min, completely stable Toxoplasma gondii
40
-
30 min, 95% activity remaining Toxoplasma gondii
55
-
30 min, 50% activity remaining Toxoplasma gondii
60
-
30 min, residual activity remaining Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Toxoplasma gondii

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH profile Toxoplasma gondii

pI Value

Organism Comment pI Value Maximum pI Value
Toxoplasma gondii chromatofocusing
-
4.7