Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is cold-active | Colwellia psychrerythraea |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Colwellia psychrerythraea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 16% inhibition at 1 mM, 24% inhibition at 10 mM | Colwellia psychrerythraea | |
DTT | 25% inhibition at 1 mM, complete inhibition at 10 mM | Colwellia psychrerythraea | |
EDTA | complete inhibition at 1-10 mM | Colwellia psychrerythraea | |
Mn2+ | complete inhibition at 10 mM | Colwellia psychrerythraea | |
Zn2+ | complete inhibition at 0.1-10 mM | Colwellia psychrerythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, -1°C | Colwellia psychrerythraea | |
0.058 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, 9°C | Colwellia psychrerythraea | |
0.072 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, 19°C | Colwellia psychrerythraea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Colwellia psychrerythraea | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 22% activation at 10 | Colwellia psychrerythraea | |
NaCl | absolutely required for activity | Colwellia psychrerythraea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
gel filtration | Colwellia psychrerythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia psychrerythraea | - |
marine psychrophile, strain 34H | - |
Purification (Comment) | Organism |
---|---|
native enzyme 460fold to homogeneity by ion exchange and hydroxy apatite chromatography, and ion exchange chromatography | Colwellia psychrerythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Colwellia psychrerythraea | protein fragments of azocasein of MW 71 kDa, 83 kDa, and 22 kDa | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | best substrate | Colwellia psychrerythraea | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-Ala-Pro-Leu-4-nitroanilide + H2O | - |
Colwellia psychrerythraea | L-Ala-Ala-Pro-Leu + 4-nitroaniline | - |
? | |
L-Arg-7-amido-4-methylcoumarin + H2O | high activity | Colwellia psychrerythraea | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Leu-7-amido-4-methylcoumarin + H2O | - |
Colwellia psychrerythraea | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
additional information | substrate specificity, no activity with L-Pro-7-amido-4-methylcoumarin and L-Asp-7-amido-4-methylcoumarin, L-Ser-7-amido-4-methylcoumarin is a poor substrate, overview | Colwellia psychrerythraea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 71000, SDS-PAGE | Colwellia psychrerythraea |
Synonyms | Comment | Organism |
---|---|---|
ColAP | - |
Colwellia psychrerythraea |
cold-active aminopeptidase | - |
Colwellia psychrerythraea |
LAPase | - |
Colwellia psychrerythraea |
More | the enzyme belongs to the M1 peptidase family | Colwellia psychrerythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is cold-active | Colwellia psychrerythraea |
20 | - |
assay at | Colwellia psychrerythraea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life is 67 min | Colwellia psychrerythraea |
40 | - |
half-life is 38 min | Colwellia psychrerythraea |
45 | - |
half-life is 10 min | Colwellia psychrerythraea |
50 | - |
half-life is 5 min | Colwellia psychrerythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, -1°C | Colwellia psychrerythraea | |
0.13 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, 9°C | Colwellia psychrerythraea | |
0.36 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, 19°C | Colwellia psychrerythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Colwellia psychrerythraea |