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Literature summary for 3.4.11.6 extracted from
- Purification, characterization, and sequencing of an extracellular cold-active aminopeptidase produced by marine psychrophile Colwellia psychrerythraea strain 34H (2004), Appl. Environ. Microbiol., 70, 3321-3328.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is cold-active | Colwellia psychrerythraea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic analysis | Colwellia psychrerythraea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 16% inhibition at 1 mM, 24% inhibition at 10 mM | Colwellia psychrerythraea |  |
| DTT | 25% inhibition at 1 mM, complete inhibition at 10 mM | Colwellia psychrerythraea | |
| EDTA | complete inhibition at 1-10 mM | Colwellia psychrerythraea | |
| Mn2+ | complete inhibition at 10 mM | Colwellia psychrerythraea | |
| Zn2+ | complete inhibition at 0.1-10 mM | Colwellia psychrerythraea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, -1°C | Colwellia psychrerythraea | |
| 0.058 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, 9°C | Colwellia psychrerythraea | |
| 0.072 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, 19°C | Colwellia psychrerythraea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Colwellia psychrerythraea | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 22% activation at 10 | Colwellia psychrerythraea | |
| NaCl | absolutely required for activity | Colwellia psychrerythraea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
gel filtration | Colwellia psychrerythraea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colwellia psychrerythraea | - |
marine psychrophile, strain 34H | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 460fold to homogeneity by ion exchange and hydroxy apatite chromatography, and ion exchange chromatography | Colwellia psychrerythraea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Colwellia psychrerythraea | protein fragments of azocasein of MW 71 kDa, 83 kDa, and 22 kDa | - |
? | |
| L-Ala-7-amido-4-methylcoumarin + H2O | best substrate | Colwellia psychrerythraea | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
| L-Ala-Ala-Pro-Leu-4-nitroanilide + H2O | - |
Colwellia psychrerythraea | L-Ala-Ala-Pro-Leu + 4-nitroaniline | - |
? | |
| L-Arg-7-amido-4-methylcoumarin + H2O | high activity | Colwellia psychrerythraea | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
| L-Leu-7-amido-4-methylcoumarin + H2O | - |
Colwellia psychrerythraea | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
| additional information | substrate specificity, no activity with L-Pro-7-amido-4-methylcoumarin and L-Asp-7-amido-4-methylcoumarin, L-Ser-7-amido-4-methylcoumarin is a poor substrate, overview | Colwellia psychrerythraea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 71000, SDS-PAGE | Colwellia psychrerythraea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ColAP | - |
Colwellia psychrerythraea |
| cold-active aminopeptidase | - |
Colwellia psychrerythraea |
| LAPase | - |
Colwellia psychrerythraea |
| More | the enzyme belongs to the M1 peptidase family | Colwellia psychrerythraea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is cold-active | Colwellia psychrerythraea |
| 20 | - |
assay at | Colwellia psychrerythraea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life is 67 min | Colwellia psychrerythraea |
| 40 | - |
half-life is 38 min | Colwellia psychrerythraea |
| 45 | - |
half-life is 10 min | Colwellia psychrerythraea |
| 50 | - |
half-life is 5 min | Colwellia psychrerythraea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, -1°C | Colwellia psychrerythraea | |
| 0.13 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, 9°C | Colwellia psychrerythraea | |
| 0.36 | - |
L-Ala-Ala-Pro-Leu-4-nitroanilide | pH 7.2, 19°C | Colwellia psychrerythraea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Colwellia psychrerythraea |
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