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Literature summary for 3.4.11.6 extracted from

  • Sanz, Y.; Toldra, F.
    Purification and characterization of an arginine aminopeptidase from Lactobacillus sakei (2002), Appl. Environ. Microbiol., 68, 1980-1987.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.6

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.1 mM, activation to 128% of control. 1.0 mM, activation to 146% of control Latilactobacillus sakei

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin 0.1 mM, 12% inhibition. 1.0 mM, 16% inhibition Latilactobacillus sakei
Ca2+ 0.1 mM CaCl2, 19% inhibition Latilactobacillus sakei
Co2+ 0.1 mM CoCl2, 23% inhibition Latilactobacillus sakei
Cu2+ 0.1 mM CuCl2, complete inhibition Latilactobacillus sakei
Hg2+ 0.1 mM HgCl2, complete inhibition Latilactobacillus sakei
iodoacetate 0.1 mM, complete inactivation Latilactobacillus sakei
MgCl2 0.1 mM MgCl2, 22% inhibition Latilactobacillus sakei
Zn2+ 0.1 mM ZnCl2, 82% inhibition Latilactobacillus sakei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0159
-
 Arg-7-amido-4-methylcoumarin pH 5.0, 37°C Latilactobacillus sakei
0.026
-
 Lys-7-amido-4-methylcoumarin pH 5.0, 37°C Latilactobacillus sakei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 3 * 60000, SDS-PAGE Latilactobacillus sakei
180000
-
 gel filtration Latilactobacillus sakei

Organism

Organism UniProt Comment Textmining
Latilactobacillus sakei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Latilactobacillus sakei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17
-
-
 Latilactobacillus sakei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-4-nitroanilide + H2O as active as Arg-7-amido-4-methylcoumarin Latilactobacillus sakei Arg + 4-nitroaniline
-
 ?
Arg-7-amido-4-methylcoumarin + H2O
-
 Latilactobacillus sakei Arg + 7-amino-4-methylcoumarin
-
 ?
Arg-Ala + H2O 82% of the activity with Arg-Arg Latilactobacillus sakei Arg + Ala
-
 ?
Arg-Arg + H2O
-
 Latilactobacillus sakei Arg + Arg
-
 ?
Arg-Asp + H2O 25% of the activity with Arg-Arg Latilactobacillus sakei Arg + Asp
-
 ?
Arg-Ile + H2O 32% of the activity with Arg-Arg Latilactobacillus sakei Arg + Ile
-
 ?
Arg-Leu + H2O 25% of the activity with Arg-Arg Latilactobacillus sakei Arg + Leu
-
 ?
Arg-Lys + H2O 88% of the activity with Arg-Arg Latilactobacillus sakei Arg + Lys
-
 ?
Arg-Phe + H2O 35% of the activity with Arg-Arg Latilactobacillus sakei Arg + Phe
-
 ?
Lys-4-nitroanilide + H2O 3.6% of the activity with Arg-7-amido-4-methylcoumarin Latilactobacillus sakei Lys + 4-nitroaniline
-
 ?
Lys-7-amido-4-methylcoumarin + H2O 4.3% of the activity with Arg-7-amido-4-methylcoumarin Latilactobacillus sakei Lys + 7-amino-4-methylcoumarin
-
 ?
Lys-Ala + H2O 88% of the activity with Arg-Arg Latilactobacillus sakei Lys + Ala
-
 ?
Lys-Lys + H2O 30% of the activity with Arg-Arg Latilactobacillus sakei Lys + Lys
-
 ?
additional information no activity with Ala-Ala, Ala-Lys, Asp-Ala, Gly-Ala and Leu-Ala. The enzyme exclusively hydrolyzes basic amino acids from the amino termini of peptide substrates. The nature of the amino acid residue at the C-terminus of the dipeptide has an effect on hydrolysis rate. The activity is maximal towards dipeptides with Arg, Lys, or Ala as the C-terminal residue Latilactobacillus sakei ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 60000, SDS-PAGE Latilactobacillus sakei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Latilactobacillus sakei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45 15°C: about 30% of maximal activity, 25°C: about 75% of maximal activity, 45°C: about 70% of maximal activity Latilactobacillus sakei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Latilactobacillus sakei

pH Range

pH Minimum pH Maximum Comment Organism
4.5 5.5 pH 4.5: about 80% of maximal activity, pH 5.5: about 25% of maximal activity, activity is negligible at pH values beyond pH 4.0 and pH 6.0 Latilactobacillus sakei