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Literature summary for 3.4.11.5 extracted from
- Secretory expression and characterization of prolyl aminopeptidase in Bacillus subtilis (2018), J. Food Sci. Biotechnol., 37, 1135-1140 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pap, recombinant overexpression in Bacillus subtilis strain WB600 from plasmid pMA5-pap, about 5fold induction with 2 mM CaCl2 and 5% D-sorbitol in TB medium | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | W8GG09 | - |
- |
| Aspergillus oryzae JN-412 | W8GG09 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Bacillus subtilis strain WB600 8.8fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 247.3 | - |
purified recombinant enzyme, pH 7.5, 50°C | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Prolyl aminopeptidase | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Aspergillus oryzae |
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Release 2023.1 (January 2023)
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