Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Streptomyces lavendulae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Streptomyces lavendulae | |
DTT | - |
Streptomyces lavendulae | |
EDTA | - |
Streptomyces lavendulae | |
PMSF | - |
Streptomyces lavendulae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enhances activity | Streptomyces lavendulae | |
Cu2+ | enhances activity | Streptomyces lavendulae | |
Mg2+ | enhances activity | Streptomyces lavendulae | |
Mn2+ | enhances activity, atomic absorption studies reveal the presence of Mn2+ in the protein as a co-factor | Streptomyces lavendulae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Streptomyces lavendulae |
240000 | - |
native PAGE | Streptomyces lavendulae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | Streptomyces lavendulae | - |
? | - |
? | |
Gly-Pro-4-nitroanilide + H2O | Streptomyces lavendulae | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae | M4MBM1 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Streptomyces lavendulae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Streptomyces lavendulae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 60000 * 4 | Streptomyces lavendulae |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase P | - |
Streptomyces lavendulae |
APP | - |
Streptomyces lavendulae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Streptomyces lavendulae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme retains 50% of activity after 30 min of incubation | Streptomyces lavendulae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces lavendulae |