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Literature summary for 3.4.11.5 extracted from

  • Mahon, C.S.; ODonoghue, A.J.; Goetz, D.H.; Murray, P.G.; Craik, C.S.; Tuohy, M.G.
    Characterization of a multimeric, eukaryotic prolyl aminopeptidase: an inducible and highly specific intracellular peptidase from the non-pathogenic fungus Talaromyces emersonii (2009), Microbiology, 155, 3673-3682.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.5

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli Rasamsonia emersonii

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.1 mM, relative activity: 6% Rasamsonia emersonii
diethylpyrocarbonate 1 mM, relative activity: 3% Rasamsonia emersonii
HgCl2 0.5 mM, relative activity: 31% Rasamsonia emersonii
iodoacetic acid 0.2 mM, relative activity: 52% Rasamsonia emersonii
N-ethylmaleimide 0.1 mM, relative activity: 71% Rasamsonia emersonii
N-tosyl-L-lysine chloromethyl ketone 1 mM, relative activity: 62% Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.101
-
 L-proline-7-amido-4-methylcoumarin
-
 Rasamsonia emersonii

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 SDS-PAGE Rasamsonia emersonii
50600
-
 calculated from cDNA Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii Q8X1C7
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity by ammonium sulfate fractionation followed by five successive chromatographic steps Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline-7-amido-4-methylcoumarin + H2O enzyme displays a 50fold specificity for cleaving N-terminal Pro-X compared with Ala-X or Val-X bonds Rasamsonia emersonii L-proline + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
hexamer gel filtration chromatography, 6 * 46000 Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
Prolyl aminopeptidase
-
 Rasamsonia emersonii
TePAP
-
 Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 enzyme retains 40% activity after 1 h and is completely inactivated after 2 h Rasamsonia emersonii
60
-
 50% activity Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
213.7
-
 L-proline-7-amido-4-methylcoumarin
-
 Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Rasamsonia emersonii

Expression

Organism Comment Expression
Rasamsonia emersonii Northern analysis indicates that Tepap mRNA levels are regulated by the composition of the growth medium. Highest Tepap transcript levels are observed when the fungus is grown in medium containing glucose and the protein hydrolysate casitone up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2100
-
 L-proline-7-amido-4-methylcoumarin
-
 Rasamsonia emersonii