Any feedback?
Literature summary for 3.4.11.5 extracted from
- Characterisation of Aspergillus niger prolyl aminopeptidase (2005), Mol. Gen. Genet., 272, 673-679.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PCMB | - |
Aspergillus niger |  |
| TLCK | - |
Aspergillus niger | |
| TPCK | - |
Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
Pro-4-nitroanilide | - |
Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
6 * 50000, SDS-PAGE | Aspergillus niger |
| 316000 | - |
gel filtration of the native enzyme | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | Q96VT2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| four chromatography steps | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.21 | - |
Pro-4-nitroanilide, cell free extract at 30°C, pH 7.5 | Aspergillus niger |
| 6.6 | - |
PLSRTLSVAAKK, at 30°C, pH 7.5 | Aspergillus niger |
| 33.4 | - |
Pro-Leu-Gly-NH2, at 30°C, pH 7.5 | Aspergillus niger |
| 55.5 | - |
PPGFSPFR, at 30°C, pH 7.5 | Aspergillus niger |
| 85.5 | - |
Pro-Ala, at 30°C, pH 7.5 | Aspergillus niger |
| 290 | - |
Pro-4-nitroanilide, four-fold purified enzyme at 30°C, pH 7.5 | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Pro + H2O | - |
Aspergillus niger | L-alanine + L-proline | - |
? | |
| L-hydroxyprolyl-2-naphthylamide + H2O | - |
Aspergillus niger | L-hydroxyproline + 2-naphthylamine | - |
? | |
| PLSRTLSVAAKK + H2O | - |
Aspergillus niger | LSRTLSVAAKK + L-proline | - |
? | |
| PPGFSPFR + H2O | - |
Aspergillus niger | PGFSPFR + L-proline | - |
? | |
| Pro-2-naphthylamide + H2O | - |
Aspergillus niger | 2-naphthylamine + L-proline | - |
? | |
| Pro-4-nitroanilide + H2O | - |
Aspergillus niger | 4-nitroaniline + L-proline | - |
? | |
| Pro-Ala + H2O | - |
Aspergillus niger | L-alanine + L-proline | - |
? | |
| Pro-Leu-Gly-NH2 + H2O | - |
Aspergillus niger | Leu-Gly-NH2 + L-proline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 50000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAP | - |
Aspergillus niger |
| Prolyl aminopeptidase | - |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
37 | significant activity decrease at 50°C | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | highest stability found between pH 6 an 7 | Aspergillus niger |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
