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Literature summary for 3.4.11.5 extracted from

  • Nakajima, Y.; Ito, K.; Sakata, M.; Xu, Y.; Nakashima, K.; Matsubara, F.; Hatakeyama, S.; Yoshimoto, T.
    Unusual extra space at the active site and high activity for acetylated hydroxyproline of prolyl aminopeptidase from Serratia marcescens (2006), J. Bacteriol., 188, 1599-1606.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Serratia marcescens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
Ala-TBODA 1 mM Serratia marcescens
Sar-TBODA 10 mM Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
 Pro-2-naphthylamide Y149A mutant enzyme Serratia marcescens
0.51
-
 Pro-2-naphthylamide wild type enzyme Serratia marcescens
0.83
-
 L-hydroxyproline-2-naphthylamide Pro-bNA, F139A mutant enzyme Serratia marcescens
0.84
-
 hydroxyproline-beta-naphthylamide F236A mutant enzyme Serratia marcescens
1.13
-
 hydroxyproline-beta-naphthylamide Y149A mutant enzyme Serratia marcescens
1.17
-
 Pro-2-naphthylamide F139A mutant enzyme Serratia marcescens
1.31
-
 Pro-2-naphthylamide F236A mutant enzyme Serratia marcescens
1.45
-
 4-acetyloxyproline-beta-naphthylamide F139A mutant enzyme Serratia marcescens
1.58
-
 Pro-2-naphthylamide C271A mutant enzyme Serratia marcescens
1.6
-
 4-acetyloxyproline-beta-naphthylamide F236A mutant enzyme Serratia marcescens
1.64
-
 4-acetyloxyproline-beta-naphthylamide wild type enzyme Serratia marcescens
2.67
-
 4-acetyloxyproline-beta-naphthylamide C271A mutant enzyme Serratia marcescens
2.92
-
 4-acetyloxyproline-beta-naphthylamide Y149A mutant enzyme Serratia marcescens
4.27
-
 hydroxyproline-beta-naphthylamide C271A mutant enzyme Serratia marcescens
9.3
-
 L-hydroxyproline-2-naphthylamide wild type enzyme Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens O32449
-
-

Purification (Commentary)

Purification (Comment) Organism
column chromatography Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.21
-
 L-hydroxyproline-2-naphthylamide, at 37°C, pH 8.0 Serratia marcescens
0.8
-
 Pro-2-naphthylamide, at 37°C, pH 8.0 Serratia marcescens
8.34
-
 4-acetyloxyproline-2-naphthylamide, at 37°C, pH 8.0 Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-acetyloxyproline-2-naphthylamide + H2O
-
 Serratia marcescens 2-naphthylamine + 4-acetyloxyproline
-
 ?
L-hydroxyproline-2-naphthylamide + H2O
-
 Serratia marcescens 2-naphthylamine + L-hydroxyproline
-
 ?
L-hydroxyprolyl-2-naphthylamide + H2O
-
 Serratia marcescens L-hydroxyproline + 2-naphthylamine
-
 ?
Pro-2-naphthylamide + H2O
-
 Serratia marcescens beta-naphthylamine + L-proline
-
 ?

Synonyms

Synonyms Comment Organism
proline iminopeptidase
-
 Serratia marcescens
Prolyl aminopeptidase
-
 Serratia marcescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 L-hydroxyproline-2-naphthylamide F236A mutant enzyme Serratia marcescens
0.03 0.55 Pro-2-naphthylamide C271A mutant enzyme Serratia marcescens
0.063
-
 L-hydroxyproline-2-naphthylamide F139A mutant enzyme Serratia marcescens
0.12
-
 L-hydroxyproline-2-naphthylamide Y149A mutant enzyme Serratia marcescens
0.35
-
 Pro-2-naphthylamide F236A mutant enzyme Serratia marcescens
0.63
-
 Pro-2-naphthylamide Y149A mutant enzyme Serratia marcescens
0.69
-
 Pro-2-naphthylamide F139A mutant enzyme Serratia marcescens
0.88
-
 4-acetyloxyproline-2-naphthylamide Y149A mutant enzyme Serratia marcescens
1.79
-
 L-hydroxyproline-2-naphthylamide C271A mutant enzyme Serratia marcescens
3.13
-
 4-acetyloxyproline-2-naphthylamide F236A mutant enzyme Serratia marcescens
3.52
-
 4-acetyloxyproline-2-naphthylamide F236A mutant enzyme Serratia marcescens
3.9
-
 L-hydroxyproline-2-naphthylamide wild type enzyme Serratia marcescens
4.93
-
 4-acetyloxyproline-2-naphthylamide C271A mutant enzyme Serratia marcescens
5.85
-
 4-acetyloxyproline-2-naphthylamide F139A mutant enzyme Serratia marcescens
7.09
-
 Pro-2-naphthylamide C271A mutant enzyme Serratia marcescens
9.7
-
 Pro-2-naphthylamide wild type enzyme Serratia marcescens
84.1
-
 4-acetyloxyproline-2-naphthylamide wild type enzyme Serratia marcescens