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Literature summary for 3.4.11.4 extracted from

  • Bhosale, M.; Pande, S.; Kumar, A.; Kairamkonda, S.; Nandi, D.
    Characterization of two M17 family members in Escherichia coli, Peptidase A and Peptidase B (2010), Biochem. Biophys. Res. Commun., 395, 76-81.
    View publication on PubMed
Search for more literature for 3.4.11.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Escherichia coli
bestatin
-
 Escherichia coli
pepstatin
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
0.14
-
 L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
0.16
-
 Gly-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
0.17
-
 L-Lys-4-nitroanilide pH 8.0, 37°C Escherichia coli
0.33
-
 L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
0.401
-
 L-Asp-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
275000
-
 SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium precipitation and Q-Sepharose column Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 value about, substrate L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin Escherichia coli
1.7
-
 value about, substrate L-Gln-7-amido-4-methylcoumarin Escherichia coli
1.7
-
 value about, substrate L-Thr-7-amido-4-methylcoumarin Escherichia coli
1.7
-
 value about, substrate L-Trp-7-amido-4-methylcoumarin Escherichia coli
2.5
-
 value about, substrate L-Tyr-7-amido-4-methylcoumarin Escherichia coli
3.3
-
 value about, substrate L-Pro-7-amido-4-methylcoumarin Escherichia coli
8.3
-
 value about, substrate Gly-7-amido-4-methylcoumarin Escherichia coli
16.7
-
 value about, substrate L-Asp-7-amido-4-methylcoumarin Escherichia coli
25
-
 value about, substrate L-Met-7-amido-4-methylcoumarin Escherichia coli
31.7
-
 value about, substrate L-Lys-4-nitroanilide Escherichia coli
35
-
 value about, substrate L-Leu-7-amido-4-methylcoumarin Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O assay at pH 8.0, 37°C Escherichia coli ?
-
 ?
Gly-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + Gly
-
 ?
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Ala-L-Ala-L-Phe
-
 ?
L-Asp-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Asp
-
 ?
L-Gln-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Gln
-
 ?
L-Leu-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Leu
-
 ?
L-Lys-4-nitroanilide + H2O assay at pH 8.0, 37°C Escherichia coli 4-nitroaniline + L-Lys
-
 ?
L-Met-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Met
-
 ?
L-Pro-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Pro
-
 ?
L-Thr-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Thr
-
 ?
L-Trp-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Trp
-
 ?
L-Tyr-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Tyr
-
 ?
oxidized insulin B chain + H2O assay at pH 8.0, 37°C Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
hexamer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
PepB
-
 Escherichia coli
peptidase B
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 melting temperature Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.7
-
 L-Asp-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
12
-
 Gly-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
14
-
 L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
14.3
-
 L-Lys-4-nitroanilide pH 8.0, 37°C Escherichia coli
14.4
-
 L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
19
-
 L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23
-
 L-Asp-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
41
-
 L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
71
-
 Gly-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
89
-
 L-Lys-4-nitroanilide pH 8.0, 37°C Escherichia coli
104
-
 L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli
112
-
 L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C Escherichia coli