Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Porphyromonas gingivalis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method with 100 mM Ches (pH 9.0), 1.1 M potassium/sodium tartrate, 200 mM lithium sulfate | Porphyromonas gingivalis |
Protein Variants | Comment | Organism |
---|---|---|
E205A | inactive | Porphyromonas gingivalis |
E205Q | inactive | Porphyromonas gingivalis |
E636A | reduced activity | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala-Ile-pyrrolidin-2-yl boronic acid | - |
Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Ala-Phe-Pro-beta-naphthylamide | wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis | |
0.38 | - |
Gly-Ala-Pro-beta-naphthylamide | mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis | |
0.42 | - |
Gly-Ala-Pro-beta-naphthylamide | wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis | |
0.85 | - |
Ala-Phe-Pro-beta-naphthylamide | mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79390 | - |
calculated from amino acid sequence | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography and Toyopearl HW65C column chromatography | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Phe-Pro-beta-naphthylamide + H2O | - |
Porphyromonas gingivalis | Ala-Phe-Pro + beta-naphthylamine | - |
? | |
Gly-Ala-Pro-beta-naphthylamide + H2O | - |
Porphyromonas gingivalis | Gly-Ala-Pro + beta-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
prolyl tripeptidyl aminopeptidase | - |
Porphyromonas gingivalis |
PTP39 | - |
Porphyromonas gingivalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
Ala-Phe-Pro-beta-naphthylamide | mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis | |
265 | - |
Gly-Ala-Pro-beta-naphthylamide | mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis | |
354 | - |
Gly-Ala-Pro-beta-naphthylamide | wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis | |
511 | - |
Ala-Phe-Pro-beta-naphthylamide | wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000881 | - |
Ala-Ile-pyrrolidin-2-yl boronic acid | wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis | |
0.0488 | - |
Ala-Ile-pyrrolidin-2-yl boronic acid | mutant enzyme E363A, in 20 mM Tris-HCl (pH 7.0) at 37°C | Porphyromonas gingivalis |