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Literature summary for 3.4.11.4 extracted from

  • Xu, Y.; Nakajima, Y.; Ito, K.; Zheng, H.; Oyama, H.; Heiser, U.; Hoffmann, T.; Gaertner, U.T.; Demuth, H.U.; Yoshimoto, T.
    Novel inhibitor for prolyl tripeptidyl aminopeptidase from Porphyromonas gingivalis and details of substrate-recognition mechanism (2008), J. Mol. Biol., 375, 708-719.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Porphyromonas gingivalis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 100 mM Ches (pH 9.0), 1.1 M potassium/sodium tartrate, 200 mM lithium sulfate Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
E205A inactive Porphyromonas gingivalis
E205Q inactive Porphyromonas gingivalis
E636A reduced activity Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
Ala-Ile-pyrrolidin-2-yl boronic acid
-
Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
Ala-Phe-Pro-beta-naphthylamide wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis
0.38
-
Gly-Ala-Pro-beta-naphthylamide mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis
0.42
-
Gly-Ala-Pro-beta-naphthylamide wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis
0.85
-
Ala-Phe-Pro-beta-naphthylamide mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79390
-
calculated from amino acid sequence Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography and Toyopearl HW65C column chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Phe-Pro-beta-naphthylamide + H2O
-
Porphyromonas gingivalis Ala-Phe-Pro + beta-naphthylamine
-
?
Gly-Ala-Pro-beta-naphthylamide + H2O
-
Porphyromonas gingivalis Gly-Ala-Pro + beta-naphthylamine
-
?

Synonyms

Synonyms Comment Organism
prolyl tripeptidyl aminopeptidase
-
Porphyromonas gingivalis
PTP39
-
Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.8
-
Ala-Phe-Pro-beta-naphthylamide mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis
265
-
Gly-Ala-Pro-beta-naphthylamide mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis
354
-
Gly-Ala-Pro-beta-naphthylamide wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis
511
-
Ala-Phe-Pro-beta-naphthylamide wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000881
-
Ala-Ile-pyrrolidin-2-yl boronic acid wild type enzyme, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis
0.0488
-
Ala-Ile-pyrrolidin-2-yl boronic acid mutant enzyme E363A, in 20 mM Tris-HCl (pH 7.0) at 37°C Porphyromonas gingivalis