Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
C25S | mutant enzyme shows no loss or decrease of enzymatic activity | Bacillus subtilis |
DELTA1-119 | inactive mutant enzyme | Bacillus subtilis |
DELTA1-159 | inactive mutant enzyme | Bacillus subtilis |
DELTA1-208 | inactive mutant enzyme | Bacillus subtilis |
DELTA1-66 | mutant enzyme requires a concentration of Zn2+ ion at least ten-fold higher to reach maximal activity without significantly affecting kinetic parameters such as Km and Vmax compared to the full length tripeptidase | Bacillus subtilis |
DELTA211-410 | inactive mutant enzyme | Bacillus subtilis |
DELTA271-410 | inactive mutant enzyme | Bacillus subtilis |
DELTA291-410 | inactive mutant enzyme | Bacillus subtilis |
DELTA361-410 | inactive mutant enzyme | Bacillus subtilis |
General Stability | Organism |
---|---|
denaturation by guanidine hydrochloride at room temperature. Zn2+ makes the enzyme become much more refractory to the denaturing effect of guanidine hydrochloride than Co2+ | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.1 mM, 90% inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
Met-Ala-Ser | pH 7.4, 55°C, in presence of 0.004 mM Zn2+ | Bacillus subtilis | |
3.5 | - |
Met-Ala-Ser | pH 7.4, 55°C, in presence of 0.004 mM Co2+ | Bacillus subtilis | |
5.1 | - |
Met-Ala-Ser | pH 7.4, 55°C, without metal | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, half-maximal activity at 1700 nM | Bacillus subtilis | |
Mn2+ | activates | Bacillus subtilis | |
Zn2+ | activates, half-maximal activity at 650 nM. The enzyme is likely to be a Zn2+-dependent metalloenzyme, N-terminal region stabilizes Zn2+-binding. Atomic absorption spectroscopy reveals 0.26 atoms of Zn2+ per molecule. When the enzyme is denatured with 6 M guanidine hydrochloride and refolded in the presence of 4 mM Zn2+, it contains 0.53 atoms of Zn2+ per molecule with 1.5fold increase in activity | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Ala-Ser + H2O | - |
Bacillus subtilis | Met + Ala-Ser | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PepT | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bacillus subtilis |