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Literature summary for 3.4.11.4 extracted from
- Purification and molecular characterization of a tripeptidase (PepT) from Lactobacillus helveticus (2000), Appl. Environ. Microbiol., 66, 794-800.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Lactobacillus helveticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, complete inhibition | Lactobacillus helveticus |  |
| 2-mercaptoethanol | 0.1 mM, 42% inhibition | Lactobacillus helveticus | |
| Cd2+ | 0.1 mM, complete inhibition | Lactobacillus helveticus | |
| Co2+ | 0.1 mM, 72% inhibition | Lactobacillus helveticus | |
| Cu2+ | 0.1 mM, complete inhibition | Lactobacillus helveticus | |
| dithiothreitol | 0.1 mM, 72% inhibition | Lactobacillus helveticus | |
| EDTA | 0.1 mM, complete inhibition | Lactobacillus helveticus | |
| EGTA | 0.1 mM, complete inhibition | Lactobacillus helveticus | |
| Mn2+ | 0.1 mM, 53% inhibition | Lactobacillus helveticus | |
| phenylmethylsulfonyl fluoride | 0.1 mM, 89% inhibition | Lactobacillus helveticus | |
| Zn2+ | 1.0 mM, 91% inhibition | Lactobacillus helveticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
Leu-Gly-Gly | - |
Lactobacillus helveticus | |
| 2.6 | - |
Met-Gly-Gly | - |
Lactobacillus helveticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation to 120% of control be 0.1 mM, activation to 168% of control by 1.0 mM | Lactobacillus helveticus | |
| Zn2+ | activation to 112% of control by 0.1 mM | Lactobacillus helveticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
3 * 47000, SDS-PAGE | Lactobacillus helveticus |
| 150000 | - |
gel filtration | Lactobacillus helveticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus helveticus | Q9L4G1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus helveticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.0567 per mg per min | Lactobacillus helveticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala-Ala + H2O | 26% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Ala + Ala-Ala | - |
? | |
| Gly-Leu-Tyr + H2O | 15% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Gly + Leu-Tyr | - |
? | |
| Leu-Gly-Gly + H2O | 34% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Leu + Gly-Gly | - |
? | |
| Leu-Leu-Ala + H2O | 33% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Leu + Leu-Ala | - |
? | |
| Leu-Leu-Leu + H2O | 15% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Leu + Leu-Leu | - |
? | |
| Lys-Gly-Gly + H2O | 13% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Lys + Gly-Gly | - |
? | |
| Met-Gly-Gly + H2O | - |
Lactobacillus helveticus | Met + Gly-Gly | - |
? | |
| Tyr-Gly-Gly + H2O | 11% of the activity with Met-Gly-Gly | Lactobacillus helveticus | Tyr + Gly-Gly | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 47000, SDS-PAGE | Lactobacillus helveticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
15 min, 55% loss of activity | Lactobacillus helveticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Lactobacillus helveticus |
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