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Literature summary for 3.4.11.4 extracted from

  • Sanz, Y.; Mulholland, F.; Toldra, F.
    Purification and Characterization of a Tripeptidase from Lactobacillus sake (1998), J. Agric. Food Chem., 46, 349-353.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Latilactobacillus sakei
2-mercaptoethanol
-
Latilactobacillus sakei
bestatin
-
Latilactobacillus sakei
dithiothreitol
-
Latilactobacillus sakei
EDTA
-
Latilactobacillus sakei
Sn2+
-
Latilactobacillus sakei
Zn2+
-
Latilactobacillus sakei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
Phe-Gly-Gly
-
Latilactobacillus sakei
13
-
Gly-Gly-Phe
-
Latilactobacillus sakei

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates Latilactobacillus sakei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
gel filtration Latilactobacillus sakei

Organism

Organism UniProt Comment Textmining
Latilactobacillus sakei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Latilactobacillus sakei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Latilactobacillus sakei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Gly-Phe + H2O
-
Latilactobacillus sakei Gly + Gly-Phe
-
?
L-Ala-L-Ala-L-Ala + H2O
-
Latilactobacillus sakei L-Ala + L-Ala-L-Ala
-
?
L-Phe-Gly-Gly + H2O
-
Latilactobacillus sakei L-Phe + Gly-Gly
-
?
Leu-Gly-Gly + H2O Leu can be replaced by Phe Latilactobacillus sakei Leu + Gly-Gly
-
?
Ser-Ser-Ser + H2O
-
Latilactobacillus sakei ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Latilactobacillus sakei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Latilactobacillus sakei