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Literature summary for 3.4.11.3 extracted from
- Insulin-regulated aminopeptidase in adipocyte is Cys-specific and affected by obesity (2015), J. Mol. Endocrinol., 55, 1-8 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Insulin | insulin effectively stimulates CysAP activity trafficking from low (LDM) density microsomes toward plasma membrane in all animal groups under study, while the insulin-stimulated traffic of LeuAP activity in adipocytes does not change as a function of metabolic challenges applied | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | high (HDM) and low (LDM) density microsomal fractions. LDM consists of microsomes without ribosomes and with portions of endoplasmatic reticulum (ER) and fragments of Golgi apparatus. HDM consists of microsomes with ribosomes adhered to its outer surface. The inner side of microsomes is biochemically equivalent to the lumen of the ER | Rattus norvegicus | - |
- |
| plasma membrane | - |
Rattus norvegicus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Rattus norvegicus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxytocin + H2O | Rattus norvegicus | - |
? | - |
? | |
| oxytocin + H2O | Rattus norvegicus Wistar | - |
? | - |
? | |
| Vasopressin + H2O | Rattus norvegicus | - |
? | - |
? | |
| Vasopressin + H2O | Rattus norvegicus Wistar | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P97629 | male | - |
| Rattus norvegicus Wistar | P97629 | male | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme in microsomal fractions | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Cys-beta-naphthylamide + H2O | - |
Rattus norvegicus | L-Cys + beta-naphthylamine | - |
? | |
| L-Cys-beta-naphthylamide + H2O | - |
Rattus norvegicus Wistar | L-Cys + beta-naphthylamine | - |
? | |
| L-cystine-di-beta-naphthylamide + 2 H2O | in the presence of thiol, the substrate is converted to L-cystine beta-naphthylamide | Rattus norvegicus | L-cystine + 2 beta-naphthylamine | - |
? | |
| L-cystine-di-beta-naphthylamide + 2 H2O | in the presence of thiol, the substrate is converted to L-cystine beta-naphthylamide | Rattus norvegicus Wistar | L-cystine + 2 beta-naphthylamine | - |
? | |
| oxytocin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| oxytocin + H2O | - |
Rattus norvegicus Wistar | ? | - |
? | |
| Vasopressin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Vasopressin + H2O | - |
Rattus norvegicus Wistar | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CysAP | - |
Rattus norvegicus |
| cystyl-aminopeptidase | - |
Rattus norvegicus |
| insulin-regulated aminopeptidase | - |
Rattus norvegicus |
| IRAP | - |
Rattus norvegicus |
| LeuAP | - |
Rattus norvegicus |
| leucyl-aminopeptidase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | IRAP is a member of the M1 family of zinc-dependent metalloproteinases | Rattus norvegicus |
| malfunction | catalytic preference and insulin-regulated traffic of IRAP in adipocytes are disturbed by obesity and are interrelated with comprehensive roles of endogenous substrates with N-terminal cysteine pair in energy metabolism homeostasis | Rattus norvegicus |
| physiological function | analysis of enzyme activity in high (HDM) and low (LDM) density microsomal fractions, and plasma membrane fraction (MF) from isolated adipocytes of healthy (C), food deprived (FD) and monosodium glutamate (MSG) obese rats on aminoacyl substrates with N-terminal Cys or Leu, in absence or presence of insulin, overview. The hydrolytic activity trafficking from LDM to MF under influence of insulin in C, MSG and FD acts only on N-terminal Cys. The pathophysiological significance of IRAP in adipocytes seems to be linked to comprehensive energy metabolism related roles of endogenous substrates with N-terminal cysteine pair such as vasopressin and oxytocin. Insulin-dependent traffic of CysAP activity has important pathophysiological implications | Rattus norvegicus |
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